Solute Carrier Family 17 (Sodium-Dependent Inorganic Phosphate Cotransporter), Member 8 (SLC17A8) (internal) antibody

Antigen: Solute Carrier Family 17 (Sodium-Dependent Inorganic Phosphate Cotransporter), Member 8 (SLC17A8)

Synonyms: Vglut3, BC042593, DFNA25, VGLUT3, vglut3, si:ch211-264e16.4

Epitope: internal

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351386

Additional Information

Alternative name: VGluT3 (Vesicular glutamate transporter 3) (internal region)

Swiss-Prot: Q7TSF2

Immunogen: A synthetic peptide from the internal region of rat VGLUT3 conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. SUBCELLULAR LOCATION: Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane. Membrane; Multi-pass membrane proteinPotential. Cell junction › synapse › synaptosome. TISSUE SPECIFICITY: Expressed in brain, kidney and liver. Expressed within the amygdala, brainstem, cerberal cortex, dorsal root ganglia, dorsal spinal cord, hippocampus, hypothalamus, retina, striatum and ventral spinal cord. Expressed within neurons of the caudate-putamen,olfactory tubercle, nucleus accumbens, hippocampus, interpeduncular nucleus and dorsal and medial raphe nuclei. Expressed in inner hair cells of the ear. Expressed at synaptic terminals within the lateral superior olive (LSO), a nucleus of the mammalian sound localization system, and in the medial nucleus of the trapezoid body (MNTB), which provides inhibitory input to the LSO. Also known as: Vesicular glutamate transporter 3, Solute carrier family 17 member 8.

Specificity: Appears to be specific for VGLUT3.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Gras, Herzog, Bellenchi et al.: "A third vesicular glutamate transporter expressed by cholinergic and serotoninergic neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 22, Issue 13, pp. 5442-51, 2002 (PubMed).

Schaefer, Varoqui, Defamie et al.: "Molecular cloning and functional identification of mouse vesicular glutamate transporter 3 and its expression in subsets of novel excitatory neurons." in: The Journal of biological chemistry, Vol. 277, Issue 52, pp. 50734-48, 2002 (PubMed).

Fremeau, Burman, Qureshi et al.: "The identification of vesicular glutamate transporter 3 suggests novel modes of signaling by glutamate." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 22, pp. 14488-93, 2002 (PubMed).

Takamori, Holt, Stenius et al.: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, 2006 (PubMed).

Seal, Akil, Yi et al.: "Sensorineural deafness and seizures in mice lacking vesicular glutamate transporter 3." in: Neuron, Vol. 57, Issue 2, pp. 263-75, 2008 (PubMed).