VP33B (C-Term) antibody

Details for Product No. ABIN351412
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Antigen
Epitope
C-Term
Reactivity
Rat (Rattus), Mouse (Murine)
Host
Rabbit
Clonality
Polyclonal
Application
Immunohistochemistry (IHC), Western Blotting (WB)
Pubmed 3 references available
Quantity 100 μL
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Immunogen A synthetic peptide from the c-terminal of rat VP33B (Vesicle protein sorting associated protein 33B) conjugated to an immunogenic carrier protein was used as the antigen. The antigen shares 91% identity with rat's and human's.
Specificity Specific for VP33B.
Purification Whole serum
Background Function: May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
Subcellular location: Late endosome membrane, Peripheral membrane protein, Cytoplasmic side. Lysosome membrane, Peripheral membrane protein, Cytoplasmic side. Note: Cytoplasmic, peripheral membrane protein associated with late endosomes/lysosomes.
Tissue specificity: Ubiquitous. Also known as: Vesicle protein sorting associated protein 33B, Vacuolar protein sorting-associated protein 33B.
Application Notes A dilution of 1: 200 to 1: 1000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Supplier Images
anti-VP33B (C-Term) antibody IF on 1 day- old mouse cortical cell culture using Rabbit antibody to c-terminal of V...
anti-VP33B (C-Term) antibody (2) IF on 1 day- old mouse cortical cell culture using Rabbit antibody to c-terminal of V...
General Gissen, Johnson, Morgan et al.: "Mutations in VPS33B, encoding a regulator of SNARE-dependent membrane fusion, cause arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome." in: Nature genetics, Vol. 36, Issue 4, pp. 400-4, 2004 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs. ..." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Pevsner, Hsu, Hyde et al.: "Mammalian homologues of yeast vacuolar protein sorting (vps) genes implicated in Golgi-to-lysosome trafficking." in: Gene, Vol. 183, Issue 1-2, pp. 7-14, 1997 (PubMed).

Validation Images
Catalog No. ABIN351412
454.67 $
Plus shipping costs $45.00
Quantity
Price
100 μL
454.67 $

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