Vacuolar Protein Sorting 33 Homolog B (Yeast) (VPS33B) (C-Term) antibody

Antigen: Vacuolar Protein Sorting 33 Homolog B (Yeast) (VPS33B)

Synonyms: FLJ14848, CG5127, DmelCG5127, dVps33B, VPS33b, MGC93106, VPS33B, MGC137868, zgc:110803, GB11157

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351412

Additional Information

Alternative name: VP33B (Vesicle protein sorting associated protein 33B) (c-terminal)

Swiss-Prot: Q63616

Immunogen: A synthetic peptide from the c-terminal of rat VP33B (Vesicle protein sorting associated protein 33B) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen shares 91% identity with rat's and human's.

Format: Lyophilized

Description: FUNCTION: May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. SUBCELLULAR LOCATION: Late endosome membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome membrane; Peripheral membrane protein; Cytoplasmic side. Note: Cytoplasmic, peripheral membrane protein associated with late endosomes/lysosomes. TISSUE SPECIFICITY: Ubiquitous. Also known as: Vesicle protein sorting associated protein 33B, Vacuolar protein sorting-associated protein 33B.

Specificity: Appears to be specific for VP33B.

Application Details

Application Notes: IHC, WB (confirmed by recombinant protein). A dilution of 1: 200 to 1: 1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

IF on 1 day- old mouse cortical cell culture using Rabbit antibody to c-terminal of VP33B (Vesicle protein sorting associated protein 33B): whole serum (ABIN351412) at 1: 500 dilution. DAPI counter stained.

IF on 1 day- old mouse cortical cell culture using Rabbit antibody to c-terminal of VP33B (Vesicle protein sorting associated protein 33B): whole serum (ABIN351412) at 1: 500 dilution. DAPI counter stained appearing in blue.

Publications

Publications: Pevsner, Hsu, Hyde et al.: "Mammalian homologues of yeast vacuolar protein sorting (vps) genes implicated in Golgi-to-lysosome trafficking." in: Gene, Vol. 183, Issue 1-2, pp. 7-14, 1997 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Gissen, Johnson, Morgan et al.: "Mutations in VPS33B, encoding a regulator of SNARE-dependent membrane fusion, cause arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome." in: Nature genetics, Vol. 36, Issue 4, pp. 400-4, 2004 (PubMed).