Vps20-Associated 1 Homolog (S. Cerevisiae) (VTA1) (Internal Region) antibody

Antigen: Vps20-Associated 1 Homolog (S. Cerevisiae) (VTA1)

Synonyms: DRG1, LIP5, SBP1, DRG-1, My012, C6orf55, HSPC228, FLJ27228, MGC84207, MGC89334

Binding Site: Internal Region

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351413

Additional Information

Alternative name: VTA1 (Vacuolar protein sorting-associated protein VTA1 homolog)

UniProt: Q9NP79

Immunogen: A synthetic peptide from the internal region of human VTA1 (Vacuolar protein sorting-associated protein VTA1 homolog) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Involved in the endosomal multivesicular bodies (MVB) pathway. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. Thought to be a cofactor of VPS4A/B, which catalyzes disassembles membrane-associated ESCRT-III assemblies. Involved in the sorting and down-regulation of EGFR. Involved in HIV-1 budding. SUBCELLULAR LOCATION: Cytoplasm. Endosome membrane; Peripheral membrane protein Also known as: Vacuolar protein sorting-associated protein VTA1 homolog, Dopamine-responsive protein DRG-1, SKD1 binding protein 1, SBP1, LYST-interacting protein 5, LIP5.

Specificity: Appears to be specific for VTA1.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Zhang, Ye, Wu et al.: "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells." in: Genome research, Vol. 10, Issue 10, pp. 1546-60, 2000 (PubMed).

Wiemann, Weil, Wellenreuther et al.: "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." in: Genome research, Vol. 11, Issue 3, pp. 422-35, 2001 (PubMed).

Mungall, Palmer, Sims et al.: "The DNA sequence and analysis of human chromosome 6." in: Nature, Vol. 425, Issue 6960, pp. 805-11, 2003 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Ward, Vaughn, Shiflett et al.: "The role of LIP5 and CHMP5 in multivesicular body formation and HIV-1 budding in mammalian cells." in: The Journal of biological chemistry, Vol. 280, Issue 11, pp. 10548-55, 2005 (PubMed).