Wolfram Syndrome 1 (Wolframin) (WFS1) antibody

Antigen: Wolfram Syndrome 1 (Wolframin) (WFS1)

Synonyms: WFS, WFRS, FLJ51211, WOLFRAMIN, DmelCG4917, CG4917, GB15257, WFS1

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351414

Additional Information

Alternative name: WFS1 (Wolframin)

Swiss-Prot: Q9JLT5

Immunogen: A synthetic peptide from the aa 100-200 of mouse WFS1 (Wolframin) conjugated to an immunogenic carrier protein was used as the immunogen. The peptide is homologous in rat.

Format: Lyophilized

Description: FUNCTION: Participates in the regulation of cellular Ca(2+) homeostasis, at least partly, by modulating the filling state of the endoplasmic reticulum Ca(2+) store. SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. Also known as: Wolframin.

Specificity: Appears to be specific for WFS1.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Inoue, Tanizawa, Wasson et al.: "A gene encoding a transmembrane protein is mutated in patients with diabetes mellitus and optic atrophy (Wolfram syndrome)." in: Nature genetics, Vol. 20, Issue 2, pp. 143-8, 1998 (PubMed).

Strom, Hoertnagel, Hofmann et al.: "Diabetes insipidus, diabetes mellitus, optic atrophy and deafness (DIDMOAD) caused by mutations in a novel gene (wolframin) coding for a predicted transmembrane protein." in: Human molecular genetics, Vol. 7, Issue 13, pp. 2021-8, 1999 (PubMed).

Takeda, Inoue, Tanizawa et al.: "WFS1 (Wolfram syndrome 1) gene product: predominant subcellular localization to endoplasmic reticulum in cultured cells and neuronal expression in rat brain." in: Human molecular genetics, Vol. 10, Issue 5, pp. 477-84, 2001 (PubMed).

Munton, Tweedie-Cullen, Livingstone-Zatchej et al.: "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 2, pp. 283-93, 2007 (PubMed).