Myc Tag antibody (AA 423-435)

Catalog No. ABIN351513

Product Details anti-Myc Tag Antibody

Target: Myc Tag

Binding Specificity: AA 423-435

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This Myc Tag antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Brand: IHC-plus™

Specificity: The antibody 9E10 may be used to detect the c-Myc tag. The c-myc gene (8q24 on human chromosome) is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase (JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. c-Myc is also essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%) Orangutan, Monkey, Marmoset, Tamarin (92%) Rat, Sheep, Hamster, Panda, Dog, Horse (85%).

Purification: Protein G purified

Immunogen: Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-myc. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Orangutan, Monkey, Marmoset, Tamarin (92%), Rat, Sheep, Hamster, Panda, Dog, Horse (85%).

Type of Immunogen: Synthetic peptide

Clone: 9E10

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (1 - 5 μg/mL), IHC, IHC-P (10 μg/mL), IP (1 - 5 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody,

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 15 mM sodium azide, approx,  pH 7.4.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for Myc Tag

Target: Myc Tag

Abstract: Myc Tag Products

Target Type: Tag

UniProt: P01106