Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) antibody

Antigen: Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1)

Synonyms: TM, EAT, MCL1L, MCL1S, Mcl-1, BCL2L3, MGC1839, MGC104264

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN351528

Additional Information

Alternative name: MCL1

Swiss-Prot: Q07820

Immunogen: A 16 amino acid peptide located near the centre of human MCL-1.

Isotype: IgG  (Matching secondary antibodies)

Description: Gene: MCL1, Standard Gene Symbol: MCL1, Synonyms: MCL1, bcl2-L-3, BCL2L3, EAT, Induced myeloid leukemia cell differentiation protein mcl-1, Mcl-1, MCL1-ES, mcl1/EAT, MCL1L, MCL1S, MGC104264, MGC1839, TM

Specificity: Recognizes an epitope within the central region (IN) of human Myeloid cell leukemia sequence 1 (MCL-1), an anti-apoptotic member of the Bcl-2 family of proteins, first identified in differentiating ML-1 myeloid cells, and expressed in many different cell lines, tissues and a variety of tumors. The MCL-1 protein, localized to intracellular membranes and particularly mitochondrial membranes, has since been isolated in two distinct isoforms, known as MCL-1 long (MCL-1L) and MCL-1 short (MCL-1S). MCL-1S has been identified as a pro-apoptotic splice variant of MCL-1L, missing the transmembrane and BH1 and BH2 (Bcl-2 homology) domains. Interaction between these two antagonistic variants can act to regulate cell survival. Recognizes both the MCL-1L (38kD) and MCL-1S (30kD) isoforms of MCL-1.

Application Details

Application Notes: IHC-P (5 µg/ml), WB (0.5 - 1 µg/ml)

Purification: Ion Exchange Chromatography

Buffer: PBS, 0.02% sodium azide.

Storage: +4°C or -20°C, Avoid repeated freezing and thawing.

Research Area: Cancer, Apoptosis/Necrosis

Restrictions: For Research Use only

Images

Human Spleen (formalin-fixed, paraffin-embedded) stained with MCL1 antibody ABIN351528 at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody ABIN481713, alkaline phosphatase-streptavidin and chromogen.