IL7R antibody (C-Term)

Catalog No. ABIN351552

Product Details anti-IL7R Antibody

Target: IL7R (Interleukin 7 Receptor (IL7R))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL7R antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: A BLAST analysis was used to suggest cross-reactivity with IL-7 from human, mouse and rat sources based on 100 % homology with the immunizing sequence. Cross-reactivity with IL-7 from other sources has not been determined

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to a region near the carboxy terminus of mouse IL-7 protein.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:500 - 1:50000), IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:10000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Expect a band approximately 55 kD in size corresponding to IL-7 in the appropriate cell lysate or extract.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Target Details for IL7R

Target: IL7R (Interleukin 7 Receptor (IL7R))

Alternative Name: IL7R / CD127 (IL7R Products)

Synonyms: IL7R antibody, CD127 antibody, IL-7Ralpha antibody, CDW127 antibody, IL-7R-alpha antibody, IL7RA antibody, ILRA antibody, IL-7RA antibody, interleukin 7 receptor antibody, IL7R antibody, Il7r antibody

Background: Name/Gene ID: IL7R
Family: Interleukin

Synonyms: IL7R, CD127, CD127 antigen, IL-7RA, IL-7 receptor subunit alpha, IL7RA, ILRA, Interleukin 7 receptor, CDW127, IL-7R subunit alpha, IL-7R-alpha

Gene ID: 3575

UniProt: P16871

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size