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CD8 antibody (APC-Cy7)

CD8 Reactivity: Mouse FACS, IHC (fro), IP Host: Rat Monoclonal 07-06-53 APC-Cy7
Catalog No. ABIN356185
  • Target See all CD8 Antibodies
    CD8
    Reactivity
    • 227
    • 72
    • 26
    • 17
    • 12
    • 12
    • 9
    • 9
    • 9
    • 9
    • 8
    • 7
    • 7
    • 6
    • 6
    • 6
    • 5
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    Mouse
    Host
    • 231
    • 54
    • 38
    Rat
    Clonality
    • 267
    • 47
    • 2
    Monoclonal
    Conjugate
    • 97
    • 40
    • 32
    • 24
    • 11
    • 8
    • 6
    • 6
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD8 antibody is conjugated to APC-Cy7
    Application
    • 272
    • 64
    • 64
    • 46
    • 45
    • 42
    • 30
    • 19
    • 17
    • 14
    • 14
    • 14
    • 8
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
    Specificity
    This antibody recognises the alpha chain of the Murine CD8 hetrerodimer (Mr. 32-34 kDa).
    Characteristics
    Synonyms: T-lymphocyte differentiation antigen T8/Leu-2, T-cell surface glycoprotein CD8 alpha chain
    Clone
    07-06-53
    Isotype
    IgG2a
    Top Product
    Discover our top product CD8 Primary Antibody
  • Application Notes
    Flow Cytometry: < / = 0.2 μg/10^6 cells. (Ref.8,9)Immunohistochemistry (Acetone-Fixed Frozen Sections) (See Ref.10)Immunoprecipitation. (Ref.8)Fractionation of CD8a+ T cells. (Ref.10)
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.1 mg/mL
    Buffer
    PBS containing 0.09 % Sodium Azide as preservative and a stabilizing agent.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
    Shelf life: one year from despatch.
    Expiry Date
    12 months
  • Target
    CD8
    Alternative Name
    CD8 (CD8 Products)
    Synonyms
    CD8 antibody, Leu2 antibody, MAL antibody, p32 antibody, CD8a molecule antibody, CD8A antibody
    Background
    In the mouse, CD8 exists in two forms: (i) a CD8 heterodimer composed of an alpha chain (CD8a/Lyt-2) and a beta chain (CD8b/Lyt-3), and (ii) a homodimer of two alpha chains. The heterodimer is found on the surface of essentially all thymocytes and the ''suppressor/cytotoxic'' subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8a without CD8b. It has been suggested that CD8+b- T cells mature extrathymically, while development of the CD8a+b+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8a+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. (Ref. 1-8)Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
    Gene ID
    12525
    UniProt
    P01731
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