Keratin 10 antibody

Catalog No. ABIN356290

Product Details anti-Keratin 10 Antibody

Target: Keratin 10 (KRT10)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 10 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognises Cytokeratin 10+13 (CK10+13). It reacts with 53 kDa and 56.6 kDa cytokeratin (CK) proteins by Western blot. With frozen sections, this antibody serves as a differentiation related marker for all stratified epithelia and stains all suprabasal cell in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.
Cellular Localization: Cytoplasmic.

Cross-Reactivity (Details): Also reacts to: KRT13

Characteristics: Synonyms: Cytokeratin-10, CK10, CK-10, Keratin-10, KRT10, KPP, K10, Keratin, Type I Cytoskeletal 10

Purification: Purified

Immunogen: Cytokeratin extraction from human ectocervical epithelium. Remarks: Molecular weight of antigen: CK10 is 56. 9 kDa and CK13 is 53 kDa.

Clone: CK208

Isotype: IgG2a

Application Details

Application Notes: Suitable for Immunohistochemistry and Immunocytochemistry (Frozen tissue sections onlyor cell smears). For IHC dilute concentared antibody at 1/50-1/100, use streptavidinapprox. biotin system orpolymer system, incubate 30 minutes at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.4 containing 1 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Storethe antibody undiluted at 2-8 °Cfor one month or (in aliquots) at -28 °Cfor longer. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for Keratin 10

Target: Keratin 10 (KRT10)

Alternative Name: Cytokeratin 10 (KRT10 Products)

Synonyms: BCIE antibody, BIE antibody, CK10 antibody, EHK antibody, K10 antibody, KPP antibody, D130054E02Rik antibody, K1C1 antibody, Krt-1.10 antibody, Krt1-10 antibody, Ka10 antibody, Ker10 antibody, KRT10 antibody, KRT25 antibody, KRT27 antibody, KRT28 antibody, keratin 10 antibody, keratin 10, type I S homeolog antibody, keratin, type I cytoskeletal 25 antibody, KRT10 antibody, Krt10 antibody, krt10.S antibody, LOC101085587 antibody

Background: Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1, CK2, CK3, CK4, CK5, CK6, CK7, CK8 and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10, CK12, CK 13, CK14, CK16, CK17, CK18, CK19 and CK20. Recently Silveira et. al. (3) have reported that in tongue squamous cell carcinoma the expression of CK 10 was significantly correlated with the presence of metasis.Synonyms: CK-10, CK10, Cytokeratin-10, K10, KPP, KRT10, Keratin, Keratin-10, Type I Cytoskeletal 10

Gene ID: 3858

UniProt: P13645