Cytokeratin 1 (KRT1) (pan) antibody

Details for Product No. ABIN356300
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Antigen
Synonyms EpK, cytok, cytokeratin, epi-keratin, epiker, xK81
Epitope
pan
(44), (7), (1), (1), (1), (1), (1), (1)
Reactivity
Cow (Bovine), Chicken, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(64), (16), (11), (6), (5), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host
Mouse
(62), (5), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(45), (28), (25), (23), (23), (19), (18), (8), (6), (2), (2), (1), (1), (1), (1)
Pubmed 2 references available
Quantity 1 mL
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Catalog No. ABIN356300
379.50 $
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Immunogen Human epidermal keratin. Remarks: Molecular weight of antigen: CK1 (67), CK2 (65. 5), CK3 (64), CK4 (59), CK5 (58)CK6 (56) CK8 (52. 5). + CK1 (67), CK2 (65. 5), CK3 (64), CK4 (59), CK5 (58) CK6 (56) CK8 (52. 5).
Clone AE1-AE3
Isotype IgG1
Specificity AE 1/AE3 represents an excellent marker for distinguishing carcinomas from non-epithelial tumors, reacts with all epithelium-derived tumors and their neoplasms. Decorates the majority of type I and type II keratins (formerly also designated cytokeratins). This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas. This antibody AE 1/AE 3 recognizes Low Molecular Weight Cytokeratins (CK 10 (56.5), CK14 (50), CK15 (50), CK16 (48) and CK19 (40) of the acidic family and CK1 (67), CK2 (65.5), CK3 (64), CK4 (59), CK5 (58) CK6 (56) and CK8 (52.5).
Cellular Localization: Cytoplasmic.Cellular Localization:
Cross-Reactivity (Details) Species reactivity (tested):Human, Monkey, Bovine, Porcine, Rat, Mouse, Rabbit and Chicken.
Characteristics Synonyms: pan Keratin, Cytokeratin Pan-reactive
Purification Protein A Chromatography.
Alternative Name Cytokeratin
Background Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1(67), CK2(65.5), CK3(64), CK4(59), CK5(58), CK6(56), CK7(54), CK8(52.5) and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10 (56.5), CK12 (56), CK13 (53), CK14 (50), CK16 (48), CK17 (46), CK18 (45), CK19 (48) and CK20 (46).Synonyms: Cytokeratin pan-reactive, pan Keratin
Research Area Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix
Application Notes Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears). For IHC dilute concentrated antibody at 1/50-1/100, use streptavidinapprox. biotin system orpolymer system, incubate 30 minutes at room temperature. For FFPE tissue sections antigen retriever like trypsin is required. Recommended Positive Control: Human skin, lung ca.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer PBS, pH 7.4, 0.05 % Sodium Azide, 1 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Expiry Date 12 months
Background publications Sun, Tseng, Huang et al.: "Monoclonal antibody studies of mammalian epithelial keratins: a review." in: Annals of the New York Academy of Sciences, Vol. 455, pp. 307-29, 1986 (PubMed).

Tseng, Jarvinen, Nelson et al.: "Correlation of specific keratins with different types of epithelial differentiation: monoclonal antibody studies." in: Cell, Vol. 30, Issue 2, pp. 361-72, 1983 (PubMed).

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