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Acidic Cytokeratin antibody

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Antigen
Reactivity
Chicken, Cow (Bovine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
17
11
5
5
4
4
4
4
3
1
1
1
Host
Mouse
15
2
Clonality (Clone)
Monoclonal ()
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
11
10
9
8
6
2
1
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Immunogen Human epithelial keratin. Remarks: Molecular weight of antigen: (CK 10 (56. 5), CK14 (50), CK15 (50), CK16 (48) andCK19 (40).
Clone AE1
Isotype IgG1
Specificity This antibody recognizes low molecular weight cytokeratins (CK 10 (56.5), CK14 (50), CK15 (50), CK16 (48) and CK19(40) of the acidic family.
Cellular Localization: Cytoplasmic.
Characteristics Synonyms: LMW Cytokeratins, Cytokeratin type I, Keratin type I
Purification Protein A Chromatography.
Background Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1, CK2, CK3, CK4, CK5, CK6, CK7, CK8 and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10, CK12, CK 13, CK14, CK16, CK17, CK18, CK19 and CK20.Synonyms: Cytokeratin type I, Keratin type I, LMW Cytokeratins
Application Notes Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears). For IHC dilute concentared antibody at 1/50-1/100, use streptavidinapprox. biotin system orpolymer system, incubate 30 minutes at room temperature. FFPE tissue section requires antigen retrieval (boiling tissue in 10 mM citrate, pH 6.0 for10-15 minutes followed by cooling for 10-15 minutes). Recommended Positive Control: Human skin, squamous cell Ca.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer PBS, pH 7.4 containing 1 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Expiry Date 12 months
Background publications Sun, Tseng, Huang et al.: "Monoclonal antibody studies of mammalian epithelial keratins: a review." in: Annals of the New York Academy of Sciences, Vol. 455, pp. 307-29, 1986 (PubMed).

Spagnolo, Michie, Crabtree et al.: "Monoclonal anti-keratin (AE1) reactivity in routinely processed tissue from 166 human neoplasms." in: American journal of clinical pathology, Vol. 84, Issue 6, pp. 697-704, 1986 (PubMed).