Acidic Cytokeratin antibody

Details for Product No. ABIN356303
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Antigen
Reactivity
Cow (Bovine), Chicken, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(1), (1), (1), (1), (1), (1), (1), (1)
Host
Mouse
(1)
Clonality (Clone)
Monoclonal ()
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(1), (1), (1), (1)
Pubmed 2 references available
Catalog no. ABIN356303
Quantity 0.5 mL
Price
286.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days

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Immunogen Human epithelial keratin. Remarks: Molecular weight of antigen: (CK 10 (56. 5), CK14 (50), CK15 (50), CK16 (48) andCK19 (40).
Clone AE1
Isotype IgG1
Characteristics Synonyms: LMW Cytokeratins, Cytokeratin type I, Keratin type I
Purification Protein A Chromatography.
Background Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeletonof epithelial tissue There are two types of Cytokeratins: the low weight, acidic type Icytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found inpairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weightcytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1, CK2, CK3,CK4, CK5, CK6, CK7, CK8 and CK9. The low molecular weight cytokeratins, which are theacidic cytokeratins, comprise subtypes CK10, CK12, CK 13, CK14, CK16, CK17, CK18, CK19and CK20.
Application Notes Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears). For IHC dilute concentared antibody at 1/50-1/100, use streptavidin~biotin system orpolymer system, incubate 30 minutes at room temperature. FFPE tissue section requires antigen retrieval (boiling tissue in 10 mM citrate, pH 6.0 for10-15 minutes followed by cooling for 10-15 minutes). Recommended Positive Control: Human skin, squamous cell Ca. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer PBS, pH 7.4 containing 1% BSA as stabilizer and 0.05% Sodium Azide as preservative.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
Expiry Date 12 months
Background publications Spagnolo, Michie, Crabtree et al.: "Monoclonal anti-keratin (AE1) reactivity in routinely processed tissue from 166 human neoplasms." in: American journal of clinical pathology, Vol. 84, Issue 6, pp. 697-704, 1986 (PubMed).

Sun, Tseng, Huang et al.: "Monoclonal antibody studies of mammalian epithelial keratins: a review." in: Annals of the New York Academy of Sciences, Vol. 455, pp. 307-29, 1986 (PubMed).

Hosts (1)
Reactivities (1), (1), (1), (1), (1), (1), (1), (1)
Applications (1), (1), (1), (1)
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