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Keratin Acidic (AE1) antibody

AE1 Reactivity: Human, Rat, Rabbit, Chicken, Mouse, Monkey, Cow, Pig IF, IHC (p), IHC (fro) Host: Mouse Monoclonal AE1 unconjugated
Catalog No. ABIN356303
  • Target See all Keratin Acidic (AE1) Antibodies
    Keratin Acidic (AE1)
    Reactivity
    • 32
    • 11
    • 9
    • 9
    • 9
    • 7
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    Human, Rat, Rabbit, Chicken, Mouse, Monkey, Cow, Pig
    Host
    • 18
    • 11
    • 3
    Mouse
    Clonality
    • 32
    Monoclonal
    Conjugate
    • 15
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Un-conjugated
    Application
    • 24
    • 24
    • 24
    • 20
    • 12
    • 3
    • 3
    • 2
    Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    This antibody recognizes low molecular weight cytokeratins (CK 10 (56.5), CK14 (50), CK15 (50), CK16 (48) and CK19(40) of the acidic family.
    Cellular Localization: Cytoplasmic.
    Characteristics
    Synonyms: LMW Cytokeratins, Cytokeratin type I, Keratin type I
    Purification
    Protein A Chromatography.
    Immunogen
    Human epithelial keratin. Remarks: Molecular weight of antigen: (CK 10 (56. 5), CK14 (50), CK15 (50), CK16 (48) andCK19 (40).
    Clone
    AE1
    Isotype
    IgG1
    Top Product
    Discover our top product AE1 Primary Antibody
  • Application Notes
    Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears). For IHC dilute concentared antibody at 1/50-1/100, use streptavidinapprox. biotin system orpolymer system, incubate 30 minutes at room temperature. FFPE tissue section requires antigen retrieval (boiling tissue in 10 mM citrate, pH 6.0 for10-15 minutes followed by cooling for 10-15 minutes). Recommended Positive Control: Human skin, squamous cell Ca.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.2 mg/mL
    Buffer
    PBS, pH 7.4 containing 1 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
    Shelf life: One year from despatch.
    Expiry Date
    12 months
  • Target
    Keratin Acidic (AE1)
    Abstract
    AE1 Products
    Background
    Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1, CK2, CK3, CK4, CK5, CK6, CK7, CK8 and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10, CK12, CK 13, CK14, CK16, CK17, CK18, CK19 and CK20.Synonyms: Cytokeratin type I, Keratin type I, LMW Cytokeratins
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