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Glial Fibrillary Acidic Protein (GFAP) antibody

Antigen

Glial Fibrillary Acidic Protein (GFAP)
Synonyms GFAP, DKFZp459C0729, MGC139638, FLJ45472, AI836096, cb345, gfapl, MGC110485, wu:fb34h11, wu:fk42c12, zgc:110485, etID36982.3, xx:af506734
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Cow (Bovine)
Application
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Catalog no. ABIN356478
Quantity 0.4 mg  (2.0 mg/ml)  (Variants)
Price 100.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Characteristics Synonyms: Glial Fibrillary Acidic Protein
Alternative name GFAP
Swiss-Prot P14136
Immunogen Purified human GFAP. Remarks: Molecular weight of antigen: 51 kDa
Cross-Reactivity Cow (Bovine), Guinea Pig, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine), Caprinae
Isotype IgG  (Matching secondary antibodies)
Description Gilial Fibrillary acidic protein (GFAP), a 51 kDa an intermediate filament protein is closelyrelated to non-epithelial family members, Vimentin, desmin and peripherin, which are allinvolved in structure and function of cell cytoskeleton. GFAP is expressed in centralnervous system (CNS) in the astrocytes cells, functioning in cellular processes e. g. cellstructure, movements, cell communication, brain processes and brain functions.
Specificity This antibody stains astrocytes, glial cells, ependymal cells and their correspondingtumors. In the peripheral nervous system , it stains Schwann cells, satellite and entericglial cells. This antibody distinguishes neoplasm of astrocytes origin from other neoplasm of CNS. Cellular Localization: Cytoplasmic. Species: Human, Bovine, Porcine, Ovine, Caprinae, Guinea Pig, Rat and Mouse. Others not tested.

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears): 1/50-1/200 usingstreptavidin~biotin system or polymer system. Immunofluorescence. Recommended Positive Control: Human brain or astrocytoma. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 2.0 mg/ml
Purification Purified
Buffer PBS, pH 7.4 containing 0.05% Sodium Azide as preservative and 1% BSA asstabilizer.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
Research Area Stem Cells, Cytoskeleton, Glia marker, Cell/Tissue Markers, Neurology
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "Glial Fibrillary Acidic Protein (GFAP)", type "Antibodies"
Hosts Mouse (151), Rabbit (82), Chicken (12), Goat (4), Guinea Pig (2)
Reactivities Human (162), Rat (Rattus) (65), Pig (Porcine) (61), Cow (Bovine) (45), Mouse (Murine) (45), Sheep (Ovine) (20), Rabbit (19), Dog (Canine) (18), Chicken (13), Guinea Pig (11), Mammalian (11), Cat (Feline) (8), Goat (3), Bird (Avian) (2), Caprinae (1), Hamster (1), Horse (Equine) (1), Monkey (1), Primate (1)
Applications Western Blotting (WB) (156), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (106), Immunofluorescence (IF) (98), Immunohistochemistry (Frozen Sections) (IHC (fro)) (61), Immunohistochemistry (IHC) (46), Immunoprecipitation (IP) (31), Flow Cytometry (FACS) (23), Immunocytochemistry (ICC) (21), ELISA (18), Enzyme Immunoassay (EIA) (16), ELISA (Detection) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Dot Blot (Dot) (1), ELISA (Capture) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Biotin (6), Cy3 (3), HRP (3), Alexa Fluor 488 (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
Epitopes pSer8 (2), C-Term (1), internal (1)