This antibody stains both free and intact LH and is useful for the classification of pituitary adenomas and tumors of the pituitary. Cellular Localization: Cytoplasmic.
LHB
Reactivity: Human
WB, ELISA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears): 1/50-1/100 usingstreptavidinapprox. biotin system or polymer system. Positive Control: Human anterior pituitary. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
2.0 mg/mL
Buffer
10 mM PO4, 150 mM NaCl, pH 7.4 containing 0.05 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Luteinizing Hormone (LH) is a tropic hormone which modulates the secretory activity of other endocrine glands. This glycoprotein hormone is produced in the anterior hypophysis of the pituitary gland. In the female an acute rise of LH the LH surge - triggers ovulation. In the male, where LH had also been called Interstitial Cell Stimulating Hormone (ICSH), it stimulates Leydig cell production of testosterone. The alpha subunits of LH, FSH, TSH, and hCG are identical, and contain 92 amino acids, the beta subunit vary. LH has a beta subunit of 121 amino acids (LHB) that confers its specific biologic action. and is responsible for interaction with the LH receptor.Synonyms: LH-B, LHB, LSH-beta, Luteinizing hormone subunit beta, Lutropin beta chain, Lutropin subunit beta