Protein C Tag (NULL) antibody

Details for Product No. ABIN356540, Supplier: Log in to see
Antigen
Reactivity
Tag
4
Host
Rabbit
3
1
Clonality
Polyclonal
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
4
2
1
1
1
1
Options
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Immunogen KLH conjugated synthetic peptide encoding the Protein-C epitope tag (CEDQVDPRLIDGK) was used as antigen.
Isotype Ig
Specificity This antibody is specific to Protein-C Tag.
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Target Type Tag
Background Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The epitope tag composed of a 12 residue peptide, EDQVDPRLIDGK , is derived from Protein-C, a vitamin K-dependent serine protease that regulates blood coagluation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. This polyclonal antibody provides a simple solution to detect the expression of a Protein-C epitope-tagged protein in cells.Synonyms: C tag, C-Tag, Protein C Epitope tag
Gene ID 5624, 5874
UniProt P04070
Application Notes ELISA: 1/5,000. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-Protein C Tag (NULL) antibody (ABIN356540) anti-Protein C Tag (NULL) antibody
Background publications Lichty, Malecki, Agnew, Michelson-Horowitz, Tan: "Comparison of affinity tags for protein purification." in: Protein expression and purification, Vol. 41, Issue 1, pp. 98-105, 2005 (PubMed).