DKK3 (N-Term) antibody

Details for Product No. ABIN357071
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms AW061014, C87148, REIC, RIG, Reic, p29
Epitope
N-Term
(11), (11), (9), (6), (6), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(79), (37), (18), (13), (12), (5), (1), (1)
Host
Rabbit
(68), (15), (7), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(63), (50), (23), (18), (10), (9), (2), (2), (2)
Pubmed 2 references available
Quantity 0.4 mL
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN357071
357.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human DKK3.
Specificity This antibody detects DKK3 at N-term.
Purification Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Alternative Name Dickkopf-3 (DKK3)
Background DKK3, like DKK1, DKK2, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed wide expression of the DKK3 transcript, with highest expression in heart, brain, and spinal cord. In situ hybridization reveals highest expression in mouse brain, eye, and heart.
Alternate names: Dickkopf-related protein 3, Dkk-3, REIC
Molecular Weight 38291 Da
Gene ID 27122
NCBI Accession NP_001018067
UniProt Q9UBP4
Application Notes ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09% (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Supplier Images
anti-DKK3 (N-Term) antibody anti-DKK3 (N-Term) antibody
anti-DKK3 (N-Term) antibody (2) anti-DKK3 (N-Term) antibody (Image 2)
anti-DKK3 (N-Term) antibody (3) anti-DKK3 (N-Term) antibody (Image 3)
Background publications Krupnik, Sharp, Jiang et al.: "Functional and structural diversity of the human Dickkopf gene family." in: Gene, Vol. 238, Issue 2, pp. 301-13, 1999 (PubMed).

Clark, Gurney, Abaya et al.: "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. ..." in: Genome research, Vol. 13, Issue 10, pp. 2265-70, 2003 (PubMed).

Validation Images
Did you look for something else?
back to top