DKK4 antibody (Dickkopf Homolog 4 (Xenopus Laevis)) (C-Term)

Details for Product anti-DKK4 Antibody No. ABIN357074, Supplier: Log in to see
Antigen
  • Dkk-4
  • DKK-4
  • RGD1563172
  • dickkopf homolog 4
  • dickkopf homolog 4 (Xenopus laevis)
  • dickkopf WNT signaling pathway inhibitor 4
  • dickkopf-related protein 4-like
  • LOC100356145
  • DKK4
  • Dkk4
  • LOC100623371
Alternatives
anti-Human DKK4 antibody for ELISA
Epitope
C-Term
45
20
10
9
5
4
4
2
2
2
1
1
1
1
Reactivity
Human
110
19
10
2
2
2
1
1
1
Host
Rabbit
86
17
16
Clonality
Polyclonal
Conjugate
This DKK4 antibody is un-conjugated
9
9
8
7
7
7
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
110
76
35
8
2
2
2
2
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human DKK4.
Isotype Ig
Specificity This antibody detects DKK4 at C-term.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Alternative Name Dickkopf-4 (DKK4) (DKK4 Antibody Abstract)
Background DKK4, like DKK1, DKK2, and DKK3, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis detected no expression of DKK4, but RT-PCR analysis detected DKK4 expression in cerebellum, T-cell, esophagus, and lung cDNA libraries. DKK4 blocks Xenopus Wnt8, Wnt3a, and Wnt2b, but not Dsh or Fz8, induction of a secondary axis in frog embryos, indicating that DKKs antagonize WNT function upstream of WNT receptors.Synonyms: Dickkopf-related protein 4, Dkk-4
Molecular Weight 24875 Da
Gene ID 27121, 9606
UniProt Q9UBT3
Pathways WNT Signaling
Application Notes ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-DKK4 antibody (Dickkopf Homolog 4 (Xenopus Laevis)) (C-Term) (ABIN357074) anti-Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) (C-Term) antibody
 image for anti-DKK4 antibody (Dickkopf Homolog 4 (Xenopus Laevis)) (C-Term) (ABIN357074) anti-Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) (C-Term) antibody (Image 2)
Background publications Yoshida, Satoh, Mitsuya, Tate: "Assignment of the human dickkopf (Xenopus) homolog 4 (DKK4) to chromosome 8p11.2-->p11.1 by fluorescence in situ hybridization." in: Cytogenetics and cell genetics, Vol. 94, Issue 1-2, pp. 88-9, 2001 (PubMed).

Krupnik, Sharp, Jiang, Robison, Chickering, Amaravadi, Brown, Guyot, Mays, Leiby, Chang, Duong, Goodearl, Gearing, Sokol, McCarthy: "Functional and structural diversity of the human Dickkopf gene family." in: Gene, Vol. 238, Issue 2, pp. 301-13, 1999 (PubMed).