GJA8 antibody (C-Term)

Catalog No. ABIN357099

Product Details anti-GJA8 Antibody

Target: GJA8 (Gap Junction Protein, alpha 8, 50kDa (GJA8))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GJA8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects Connexin 50 (GJA8) at C-term.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human GJA8.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for GJA8

Target: GJA8 (Gap Junction Protein, alpha 8, 50kDa (GJA8))

Alternative Name: GJA8 / Cx50 (GJA8 Products)

Synonyms: Aey5 antibody, Cnx50 antibody, Cx50 antibody, Lop10 antibody, CAE antibody, CAE1 antibody, CTRCT1 antibody, CX50 antibody, CZP1 antibody, MP70 antibody, Cx45.6 antibody, gap junction protein, alpha 8 antibody, gap junction protein alpha 8 antibody, Gja8 antibody, GJA8 antibody

Background: GJA8 is a an integral membrane protein that belongs to the connexin family, alpha-type (group II) subfamily. One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. A connexon is composed of a hexamer of connexins. This particular connexin only forms junctional channels. GJA8 is expressed in the eye lens, and defects in GJA8 are the cause of zonular pulverulent cataract type 1 (CZP1), a form of autosomal dominant congenital cataract.Synonyms: Connexin-50, Gap junction alpha-8 protein, Lens fiber protein MP70

Molecular Weight: 48097 Da

Gene ID: 2703, 9606

UniProt: P48165