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OCT4 antibody

POU5F1 Reactivity: Human WB, IHC (p), EIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN357429
  • Target See all OCT4 (POU5F1) Antibodies
    OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
    Reactivity
    • 120
    • 60
    • 30
    • 7
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 108
    • 25
    • 1
    • 1
    Rabbit
    Clonality
    • 99
    • 35
    Polyclonal
    Conjugate
    • 96
    • 6
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This OCT4 antibody is un-conjugated
    Application
    • 113
    • 61
    • 32
    • 21
    • 17
    • 17
    • 15
    • 14
    • 13
    • 11
    • 9
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
    Specificity
    This antibody detects OCT4 (OCT3).
    Purification
    Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
    Immunogen
    This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding E125 of human OCT3/4.
    Isotype
    Ig Fraction
    Top Product
    Discover our top product POU5F1 Primary Antibody
  • Application Notes
    ELISA 1: 1,000. Western blot 1: 50 - 1: 100. Immunofluorescence 1: 10 - 1: 50.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.25 mg/mL
    Buffer
    PBS with 0.09 % (W/V) sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
  • Target
    OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
    Alternative Name
    OCT3/4 (POU5F1 Products)
    Synonyms
    OCT3 antibody, OCT4 antibody, OTF-3 antibody, OTF3 antibody, OTF4 antibody, Oct-3 antibody, Oct-4 antibody, POU5F1 antibody, gp9 antibody, gp-9 antibody, oct4 antibody, pou2 antibody, POU-2 antibody, cb197 antibody, spg/pou2 antibody, chunp6868 antibody, wu:fd18d06 antibody, NF-A3 antibody, Oct-3/4 antibody, Oct3 antibody, Oct3/4 antibody, Oct4 antibody, Otf-3 antibody, Otf-4 antibody, Otf3 antibody, Otf3-rs7 antibody, Otf3g antibody, Otf4 antibody, POU class 5 homeobox 1 antibody, POU domain, class 5, transcription factor 3 antibody, POU domain, class 5, transcription factor 1 antibody, POU5F1 antibody, pou5f3 antibody, Pou5f1 antibody
    Background
    Transcription factors containing the POU homeodomain have been shown to be important regulators of tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development. Two forms of OCT3 mRNA are expressed in adult tissues as a result of alternative splicing--OCT3A and OCT3B. Reverse transcriptase PCR showed low level of expression in both OCT3A and OCT3B mRNA in all adult human tissues examined. Oct3 is present in mouse oocytes before and after fertilization. When fertilized oocytes were injected with antisense Oct3 oligonucleotides or double-stranded DNA containing the octamer motif, embryonic DNA synthesis was inhibited and the embryos were arrested at the one-cell stage.Synonyms: OCT3, OCT4, OTF3, Oct-3, Oct-4, Octamer-binding transcription factor 3, POU domain, POU5F1, class 5, transcription factor 1
    Molecular Weight
    38562 Da
    Gene ID
    5460, 9606
    UniProt
    Q01860
    Pathways
    Stem Cell Maintenance
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