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Ubiquitin Specific Peptidase 5 (USP5) (C-Term) antibody

Details for Product No. ABIN357529, Supplier: Log in to see
Antigen
  • ISOT
  • AA407472
  • ISOT-1
  • Ucht
Epitope
C-Term
17
3
3
3
2
2
2
2
1
1
1
1
1
Reactivity
Human
54
6
5
1
1
1
Host
Rabbit
36
18
2
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
48
26
9
8
4
2
Supplier
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human USP5.
Isotype Ig
Specificity This antibody is specific to USP5/ISOT (C-term).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name USP5 / ISOT (USP5 Antibody Abstract)
Background Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.Synonyms: Deubiquitinating enzyme 5, Isopeptidase T, Ubiquitin carboxyl-terminal hydrolase 5, Ubiquitin thioesterase 5, Ubiquitin-specific-processing protease 5
Molecular Weight 95786 Da
Gene ID 8078, 5874
UniProt P45974
Pathways
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-Ubiquitin Specific Peptidase 5 (USP5) (C-Term) antibody (ABIN357529) anti-Ubiquitin Specific Peptidase 5 (USP5) (C-Term) antibody
Background publications Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Ansari-Lari, Muzny, Lu et al.: "A gene-rich cluster between the CD4 and triosephosphate isomerase genes at human chromosome 12p13." in: Genome research, Vol. 6, Issue 4, pp. 314-26, 1996 (PubMed).

Falquet, Paquet, Frutiger et al.: "A human de-ubiquitinating enzyme with both isopeptidase and peptidase activities in vitro." in: FEBS letters, Vol. 359, Issue 1, pp. 73-7, 1995 (PubMed).

Falquet, Paquet, Frutiger et al.: "cDNA cloning of a human 100 kDa de-ubiquitinating enzyme: the 100 kDa human de-ubiquitinase belongs to the ubiquitin C-terminal hydrolase family 2 (UCH2)." in: FEBS letters, Vol. 376, Issue 3, pp. 233-7, 1996 (PubMed).