USP7 antibody (Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated)) (C-Term)

Details for Product anti-USP7 Antibody No. ABIN357533, Supplier: Log in to see
Antigen
  • CG1490
  • Dmel\\CG1490
  • USP7
  • 2210010O09Rik
  • AA409944
  • AA617399
  • AU019296
  • AW548146
  • C80752
  • Hausp
  • HAUSP
  • TEF1
  • UBP
  • ubiquitin-specific protease 7
  • ubiquitin specific peptidase 7 (herpes virus-associated)
  • ubiquitin specific peptidase 7
  • Usp7
  • usp7
  • USP7
Epitope
C-Term
32
24
20
15
13
8
7
6
5
5
5
4
3
2
2
2
1
1
1
1
1
Reactivity
Human
208
45
42
16
12
10
7
6
4
4
3
3
3
2
2
1
1
1
1
1
Host
Rabbit
111
97
1
Clonality
Polyclonal
Conjugate
This USP7 antibody is un-conjugated
8
8
8
4
4
4
4
4
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
181
65
64
50
48
32
13
13
7
4
3
3
1
Options
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human USP7.
Isotype Ig
Specificity This antibody is specific to USP7/HAUSP (C-term). Predicted to cross react with Mouse (100 % Antigen Homology).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name USP7 / HAUSP (USP7 Antibody Abstract)
Background Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.Synonyms: Deubiquitinating enzyme 7, Herpesvirus-associated ubiquitin-specific protease, Ubiquitin carboxyl-terminal hydrolase 7, Ubiquitin thioesterase 7, Ubiquitin-specific-processing protease 7
Molecular Weight 128271 Da
Gene ID 7874, 5874
UniProt Q93009
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7) (C-Term) antibody (ABIN357533) anti-Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7) (C-Term) antibody
 image for anti-Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7) (C-Term) antibody (ABIN357533) anti-Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7) (C-Term) antibody (Image 2)
Background publications Puente, Sánchez, Overall, López-Otín: "Human and mouse proteases: a comparative genomic approach." in: Nature reviews. Genetics, Vol. 4, Issue 7, pp. 544-58, 2003 (PubMed).

Li, Chen, Shiloh, Luo, Nikolaev, Qin, Gu: "Deubiquitination of p53 by HAUSP is an important pathway for p53 stabilization." in: Nature, Vol. 416, Issue 6881, pp. 648-53, 2002 (PubMed).

DAndrea, Pellman: "Deubiquitinating enzymes: a new class of biological regulators." in: Critical reviews in biochemistry and molecular biology, Vol. 33, Issue 5, pp. 337-52, 1999 (PubMed).

Everett, Meredith, Orr, Cross, Kathoria, Parkinson: "A novel ubiquitin-specific protease is dynamically associated with the PML nuclear domain and binds to a herpesvirus regulatory protein." in: The EMBO journal, Vol. 16, Issue 3, pp. 566-77, 1997 (PubMed).

Everett, Meredith, Orr, Cross, Kathoria, Parkinson: "A novel ubiquitin-specific protease is dynamically associated with the PML nuclear domain and binds to a herpesvirus regulatory protein." in: The EMBO journal, Vol. 16, Issue 7, pp. 1519-30, 1997 (PubMed).

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