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LLGL1+2 (pSer650), (pSer654) antibody

Details for Product No. ABIN357637
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Antigen
Epitope
pSer650, pSer654
(2), (2), (1), (1)
Reactivity
Human
(3)
Host
Rabbit
(3)
Clonality
Polyclonal
Application
ELISA, Western Blotting (WB)
(3), (1)
Pubmed 8 references available
Quantity 0.4 mL
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
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Catalog No. ABIN357637
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Immunogen KLH conjugated synthetic diphosphopeptide corresponding to amino acid residues surrounding Ser650 and Ser654 of Human LLGL1/2.
Purification Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Background LLGL1 is a protein that is similar to a tumor suppressor in Drosophila. The protein is part of a cytoskeletal network and is associated with nonmuscle myosin II heavy chain and a kinase that specifically phosphorylates this protein at serine residues. The gene for LLGL1 is located within the Smith-Magenis syndrome region on chromosome 17. LLGL2 is a protein similar to lethal (2) giant larvae of Drosophila. In fly, the protein's ability to localize cell fate determinants is regulated by the atypical protein kinase C (aPKC). In human, this protein interacts with aPKC-containing complexes and is cortically localized in mitotic cells.
Alternate names: DLG4, HGL, HUGL, Hugl-1, Human homolog to the D-lgl gene protein, LLGL, LLGL1, LLGL2, Lethal(2) giant larvae protein homolog 1, Lethal(2) giant larvae protein homolog 2
Molecular Weight 115042 Da and 113448 Da
Gene ID 3996
NCBI Accession NP_004131
UniProt Q15334
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09% (w/v) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Supplier Images
anti-LLGL1+2 (pSer650), (pSer654) antibody anti-LLGL1+2 (pSer650), (pSer654) antibody
Background publications Ludford-Menting, Thomas, Crimeen et al.: "A functional interaction between CD46 and DLG4: a role for DLG4 in epithelial polarization." in: The Journal of biological chemistry, Vol. 277, Issue 6, pp. 4477-84, 2002 (PubMed).

Bi, Yan, Stankiewicz et al.: "Genes in a refined Smith-Magenis syndrome critical deletion interval on chromosome 17p11.2 and the syntenic region of the mouse." in: Genome research, Vol. 12, Issue 5, pp. 713-28, 2002 (PubMed).

Katoh, Katoh: "Identification and characterization of human LLGL4 gene and mouse Llgl4 gene in silico." in: International journal of oncology, Vol. 24, Issue 3, pp. 737-42, 2004 (PubMed).

Grifoni, Garoia, Schimanski et al.: "The human protein Hugl-1 substitutes for Drosophila lethal giant larvae tumour suppressor function in vivo." in: Oncogene, Vol. 23, Issue 53, pp. 8688-94, 2004 (PubMed).

Yasumi, Sakisaka, Hoshino et al.: "Direct binding of Lgl2 to LGN during mitosis and its requirement for normal cell division." in: The Journal of biological chemistry, Vol. 280, Issue 8, pp. 6761-5, 2005 (PubMed).

Schimanski, Schmitz, Kashyap et al.: "Reduced expression of Hugl-1, the human homologue of Drosophila tumour suppressor gene lgl, contributes to progression of colorectal cancer." in: Oncogene, Vol. 24, Issue 19, pp. 3100-9, 2005 (PubMed).

Sutton, Gay, Edmunds et al.: "Modelling the force of infection for hepatitis B and hepatitis C in injecting drug users in England and Wales." in: BMC infectious diseases, Vol. 6, pp. 93, 2006 (PubMed).

Solinet, Akpovi, Garcia et al.: "Myosin IIB deficiency in embryonic fibroblasts affects regulators and core members of the par polarity complex." in: Histochemistry and cell biology, Vol. 136, Issue 3, pp. 245-66, 2011 (PubMed).

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