Peroxisomal Biogenesis Factor 19 (PEX19) (N-Term) antibody

Antigen: Peroxisomal Biogenesis Factor 19 (PEX19)

Synonyms: PXF, HK33, PMP1, PMPI, PXMP1, D1S2223E, FLJ55296, BEST:GH03076, DmelCG5325

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN357719

Additional Information

Characteristics: Synonyms: Peroxin-19, HK33, PXF, Peroxisomal biogenesis factor 19, Peroxisomal farnesylated protein,33 kDa housekeeping protein

Alternative name: Peroxin 19 / PEX19

Swiss-Prot: P40855

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated syntheticpeptide selected from the N-terminal region of human PXF.

Cross-Reactivity: Human, Mouse (Murine)

Isotype: Ig  (Matching secondary antibodies)

Description: The covalent attachment of prenyl lipids, such as farnesyl or geranylgeranyl groups, byspecific transferases is indispensable for the cellular sorting of many proteins. Peroxisomal farnesylated protein or PXF, which localizes to the outer surface ofperoxisomes, is 93% identical in sequence to human HK33, a predicted 299 amino acidprotein. The ubiquitous transcription of the HK33 gene indicates its role as a housekeepinggene. At least four variants of HK33 have been identified, two of which are farnesylated invitro. Evidence suggests that PXF is involved in the process of peroxisomal biogenesis orassembly. Complementation group J (CG-J) from patients with a peroxisome biogenesisdisorder (PBD), such as Zellweger syndrome, has been identified. Human PXF expressionrestored peroxisomal protein import in fibroblasts from a patient with Zellweger syndromeof CG-J. This patient was homozygous for a point mutation in PXF. These results indicatethat PXF is the causative gene for CG-J PBD and suggests that the C-terminal part of the PXFprotein, including the CAAX homology box, is required for function.

Specificity: This antibody detects PXF (N-term). Species: Human and Mouse.

Application Details

Application Notes: ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: PBS with 0.09% (W/V) Sodium Azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Caution: This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Restrictions: For Research Use only