CBX4 antibody (C-Term)

Catalog No. ABIN357751

Product Details anti-CBX4 Antibody

Target: CBX4 (Chromobox Homolog 4 (CBX4))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CBX4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody is specific to CBX4 (C-term).

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human CBX4.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western Blot: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for CBX4

Target: CBX4 (Chromobox Homolog 4 (CBX4))

Alternative Name: CBX4 / PC2 (CBX4 Products)

Synonyms: MPc2 antibody, PC2 antibody, NBP16 antibody, XPolycomb antibody, cbx4-a antibody, im:6911625 antibody, zgc:158588 antibody, chromobox 4 antibody, chromobox 4 S homeolog antibody, chromobox homolog 4 antibody, chromobox homolog 4 (Pc class homolog, Drosophila) antibody, CBX4 antibody, Cbx4 antibody, cbx4.S antibody, cbx4 antibody

Background: Chromobox homolog 4 (CBX4 or Pc2) is a member of Drosophila Polycomb group gene family. The polycomb group (PcG) genes are essential for maintenance of appropriate expression patterns of developmental master regulators and thus are essential for proper development. Changes in expression pf PcG proteins have been associated with cancer. CBX4 is a part of the cellular memory system that is responsible for the inheritance of gene activity by progeny cells. CBX4 is involved in maintaining the transcriptionally repressive state of genes. It modifies chromatin, rendering it heritably changed in its expressibility. Structurally, CBX4 contains 1 chromo domain. It was reported that Pc2 is involved in sumoylation process by recruiting SUMO E1 and E2, and Pc2 is actually SUMO E3 during sumoylation events. CBX4 expression is ubiquitous.Synonyms: Chromobox protein homolog 4, E3 SUMO-protein ligase CBX4, Polycomb 2 homolog

Molecular Weight: 61228 Da

Gene ID: 8535, 9606

UniProt: O00257