Collagen Type IX (Chain alpha 1) antibody

Details for Product No. ABIN357919, Supplier: Log in to see
Antigen
Epitope
Chain alpha 1
Reactivity
Human
4
4
3
3
3
2
1
1
1
1
1
Host
Rabbit
4
4
Clonality
Polyclonal
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
5
4
4
3
3
3
2
1
1
1
Options
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human COL9A1.
Isotype Ig
Specificity This antibody detects COL9A1 (Center). Predicted to cross react with Mouse (100 % Antigen Homology).
Purification Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Background COL9A1 is one of the three alpha chains of type IX collagen, which is a minor (5-20 % ) collagen component of hyaline cartilage. Type IX collagen is usually found in tissues containing type II collagen, a fibrillar collagen. Studies in knockout mice have shown that synthesis of the alpha 1 chain is essential for assembly of type IX collagen molecules, a heterotrimeric molecule,and that lack of type IX collagen is associated with early onset osteoarthritis. Mutations in the COL9A1 gene are associated with osteoarthritis in humans, with multiple epiphyseal dysplasia, 6, a form of chondrodysplasia, and with Stickler syndrome, a disease characterized by ophthalmic, orofacial, articular, and auditory defects.Synonyms: COL9A1
Molecular Weight 91855 Da
Gene ID 1297, 9606
UniProt P20849
Research Area Extracellular Matrix
Application Notes ELISA: 1/1,000. Western Blot: 1/50-1/100. Immunohistochemistry: 1/10-1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-Collagen Type IX (Chain alpha 1) antibody (ABIN357919) anti-Collagen Type IX (Chain alpha 1) antibody
 image for anti-Collagen Type IX (Chain alpha 1) antibody (ABIN357919) anti-Collagen Type IX (Chain alpha 1) antibody (Image 2)
Background publications Fresquet, Jowitt, Ylöstalo, Coffey, Meadows, Ala-Kokko, Thornton, Briggs: "Structural and functional characterization of recombinant matrilin-3 A-domain and implications for human genetic bone diseases." in: The Journal of biological chemistry, Vol. 282, Issue 48, pp. 34634-43, 2007 (PubMed).

Liu, Jin, Cao, Zhao, Lin, Sun: "[Analysis of association between COL9A1 gene and idiopathic congenital talipes equinovarus]." in: Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji, Vol. 29, Issue 4, pp. 427-32, 2007 (PubMed).

Van Camp, Snoeckx, Hilgert, van den Ende, Fukuoka, Wagatsuma, Suzuki, Smets, Vanhoenacker, Declau, Van de Heyning, Usami: "A new autosomal recessive form of Stickler syndrome is caused by a mutation in the COL9A1 gene." in: American journal of human genetics, Vol. 79, Issue 3, pp. 449-57, 2006 (PubMed).

Sivakumaran, Resendes, Robertson, Giersch, Morton: "Characterization of an abundant COL9A1 transcript in the cochlea with a novel 3' UTR: Expression studies and detection of miRNA target sequence." in: Journal of the Association for Research in Otolaryngology : JARO, Vol. 7, Issue 2, pp. 160-72, 2006 (PubMed).