SRSF2 antibody (serine/arginine-Rich Splicing Factor 2) (N-Term)

Details for Product anti-SRSF2 Antibody No. ABIN357967, Supplier: Log in to see
Antigen
  • PR264
  • SC-35
  • SC35
  • SFRS2
  • SFRS2A
  • SRp30b
  • D11Wsu175e
  • MRF-1
  • Pr264
  • Sfrs10
  • Sfrs2
  • pr264
  • sc-35
  • sc35
  • sfrs2
  • sfrs2a
  • srp30b
  • serine/arginine-rich splicing factor 2
  • SRSF2
  • Srsf2
  • srsf2
Epitope
N-Term
4
4
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Human
33
12
9
4
3
3
3
3
2
2
2
2
2
Host
Rabbit
28
5
Clonality
Polyclonal
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
28
12
6
5
5
5
5
2
1
1
Options
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human SFRS2.
Isotype Ig
Specificity This antibody detects SFRS2 (N-term).
Purification Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name SFRS2 / SC-35 (SRSF2 Antibody Abstract)
Background SFRS2 is necessary for the splicing of pre-mRNA. The protein is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. And it interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. It binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3' and can bind to beta-globin mRNA and commit it to the splicing pathway.Synonyms: Nuclear Speckle Marker, PR264, Splicing component 35 kDa, Splicing factor SC35, Splicing factor arginine/serine-rich 2
Molecular Weight 25476 Da (splicing protein is 35 KD).
Gene ID 6427, 9606
UniProt Q01130
Application Notes ELISA: 1/1,000. Western Blot: 1/50-1/100. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-serine/arginine-Rich Splicing Factor 2 (SRSF2) (N-Term) antibody (ABIN357967) anti-serine/arginine-Rich Splicing Factor 2 (SRSF2) (N-Term) antibody
 image for anti-serine/arginine-Rich Splicing Factor 2 (SRSF2) (N-Term) antibody (ABIN357967) anti-serine/arginine-Rich Splicing Factor 2 (SRSF2) (N-Term) antibody (Image 2)
Background publications Merdzhanova, Edmond, De Seranno, Van den Broeck, Corcos, Brambilla, Brambilla, Gazzeri, Eymin: "E2F1 controls alternative splicing pattern of genes involved in apoptosis through upregulation of the splicing factor SC35." in: Cell death and differentiation, Vol. 15, Issue 12, pp. 1815-23, 2008 (PubMed).

Solis, Peng, Crawford, Phillips, Patton: "Growth hormone deficiency and splicing fidelity: two serine/arginine-rich proteins, ASF/SF2 and SC35, act antagonistically." in: The Journal of biological chemistry, Vol. 283, Issue 35, pp. 23619-26, 2008 (PubMed).

Donev, Newall, Thome, Sheer: "A role for SC35 and hnRNPA1 in the determination of amyloid precursor protein isoforms." in: Molecular psychiatry, Vol. 12, Issue 7, pp. 681-90, 2007 (PubMed).

Sureau, Soret, Vellard, Crochet, Perbal: "The PR264/c-myb connection: expression of a splicing factor modulated by a nuclear protooncogene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 24, pp. 11683-7, 1993 (PubMed).

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