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RFC5 antibody (Middle Region)

RFC5 Reactivity: Human WB, EIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN357979
  • Target See all RFC5 Antibodies
    RFC5 (Replication Factor C (Activator 1) 5, 36.5kDa (RFC5))
    Binding Specificity
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Middle Region
    Reactivity
    • 21
    • 11
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 16
    • 3
    • 2
    Rabbit
    Clonality
    • 19
    • 2
    Polyclonal
    Conjugate
    • 14
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This RFC5 antibody is un-conjugated
    Application
    • 13
    • 6
    • 4
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Enzyme Immunoassay (EIA)
    Specificity
    This antibody detects RFC5 (Center).
    Purification
    Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
    Immunogen
    This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human RFC5.
    Isotype
    Ig Fraction
    Top Product
    Discover our top product RFC5 Primary Antibody
  • Application Notes
    ELISA: 1/1,000. Western Blot: 1/50-1/100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.25 mg/mL
    Buffer
    PBS with 0.09 % (W/V) Sodium Azide as preservative.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • Target
    RFC5 (Replication Factor C (Activator 1) 5, 36.5kDa (RFC5))
    Alternative Name
    RFC5 / RFC36 (RFC5 Products)
    Synonyms
    zgc:110313 antibody, 2610020K06Rik antibody, 2610209F07Rik antibody, 36.5kDa antibody, 36kDa antibody, Recc5 antibody, RFC36 antibody, replication factor C (activator 1) 5 antibody, replication factor C subunit 5 antibody, replication factor C subunit 5 L homeolog antibody, rfc5 antibody, Rfc5 antibody, RFC5 antibody, rfc5.L antibody
    Background
    The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. RFC5 is the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system.Synonyms: Activator 1 36 kDa subunit, Activator 1 subunit 5, RF-C 36 kDa subunit, Replication factor C 36 kDa subunit, Replication factor C subunit 5
    Molecular Weight
    38497 Da
    Gene ID
    5985, 9606
    UniProt
    P40937
    Pathways
    Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA
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