beta-Site APP-Cleaving Enzyme 1 (BACE) (pSer498) antibody

Details for Product No. ABIN358083
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms BACE1, MGC145931, bace2, MGC68881, MGC68482, BACE2, C76936, Bace, ASP2, BACE, HSPC104, zgc:77409
Epitope
pSer498
(22), (18), (17), (10), (8), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(146), (71), (49), (25), (24), (24), (19), (12), (1), (1), (1)
Host
Rabbit
(136), (9), (6)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(94), (57), (39), (29), (20), (15), (7), (6), (6), (2), (1), (1), (1)
Pubmed 5 references available
Quantity 0.4 mL
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN358083
357.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S498 of human BACE.
Specificity This antibody detects BACE1 pSer498.
Purification Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name BACE1
Background Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is BACE. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi.
Alternate names: ASP2, Aspartyl protease 2, BACE, BACE-1, Beta-secretase 1, Beta-site APP cleaving enzyme 1, Beta-site amyloid precursor protein cleaving enzyme 1, KIAA1149, Memapsin-2, Membrane-associated aspartic protease 2
Molecular Weight 55764 Da
Gene ID 23621, 9606
UniProt P56817
Research Area Proteolysis / Ubiquitin, Alzheimer's Disease, Enzymes
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09% (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Supplier Images
anti-beta-Site APP-Cleaving Enzyme 1 (BACE) (pSer498) antibody anti-beta-Site APP-Cleaving Enzyme 1 (BACE) (pSer498) antibody
anti-beta-Site APP-Cleaving Enzyme 1 (BACE) (pSer498) antibody (2) anti-beta-Site APP-Cleaving Enzyme 1 (BACE) (pSer498) antibody (Image 2)
Background publications Huang, Chang, Koelsch et al.: "Internalization of exogenously added memapsin 2 (beta-secretase) ectodomain by cells is mediated by amyloid precursor protein." in: The Journal of biological chemistry, Vol. 279, Issue 36, pp. 37886-94, 2004 (PubMed).

Chiocco, Kulnane, Younkin et al.: "Altered amyloid-beta metabolism and deposition in genomic-based beta-secretase transgenic mice." in: The Journal of biological chemistry, Vol. 279, Issue 50, pp. 52535-42, 2004 (PubMed).

Yang, Chai, Mosior et al.: "Biochemical and kinetic characterization of BACE1: investigation into the putative species-specificity for beta- and beta'-cleavage sites by human and murine BACE1." in: Journal of neurochemistry, Vol. 91, Issue 6, pp. 1249-59, 2004 (PubMed).

He, Li, Chang et al.: "GGA proteins mediate the recycling pathway of memapsin 2 (BACE)." in: The Journal of biological chemistry, Vol. 280, Issue 12, pp. 11696-703, 2005 (PubMed).

Xie, Guo: "PAR-4 is involved in regulation of beta-secretase cleavage of the Alzheimer amyloid precursor protein." in: The Journal of biological chemistry, Vol. 280, Issue 14, pp. 13824-32, 2005 (PubMed).

Validation Images
Did you look for something else?
back to top