Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pSer124) antibody

Details for Product No. ABIN358093
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Antigen
Synonyms cdc25a2, cdc25A, sb:cb317, CDC25A2, D9Ertd393e
Epitope
pSer124
(34), (28), (27), (22), (17), (15), (14), (13), (10), (9), (8), (6), (6), (6), (6), (6), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(358), (161), (158), (73), (61), (37), (12), (1), (1), (1), (1)
Host
Rabbit
(332), (29), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(16), (16), (16), (9), (9), (9), (7), (7), (7), (7), (7), (7), (7), (7), (2)
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(280), (122), (83), (70), (55), (48), (10), (4), (3), (2), (2), (2)
Pubmed 6 references available
Quantity 0.4 mL
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Catalog No. ABIN358093
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S124 of human CDC23A
Specificity This antibody detects CDC25A pSer124.
Purification Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name CDC25A
Background CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.
Alternate names: CDC-25A, Cell Division Cycle 25A, Dual specificity phosphatase Cdc25A, M-phase inducer phosphatase 1
Molecular Weight 59087 Da
Gene ID 993
NCBI Accession 9606
UniProt P30304
Research Area Cell Cycle, Cancer, Kinases/Phosphatases
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09% (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Supplier Images
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pSer124) antibody anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pSer124) antibody
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pSer124) antibody (2) anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pSer124) antibody (Image 2)
Background publications Goloudina, Yamaguchi, Chervyakova et al.: "Regulation of human Cdc25A stability by Serine 75 phosphorylation is not sufficient to activate a S phase checkpoint." in: Cell cycle (Georgetown, Tex.), Vol. 2, Issue 5, pp. 473-8, 2003 (PubMed).

Chow, Siu, Fung et al.: "DNA damage during the spindle-assembly checkpoint degrades CDC25A, inhibits cyclin-CDC2 complexes, and reverses cells to interphase." in: Molecular biology of the cell, Vol. 14, Issue 10, pp. 3989-4002, 2003 (PubMed).

Chen, Ryan, Piwnica-Worms: "Chk1 kinase negatively regulates mitotic function of Cdc25A phosphatase through 14-3-3 binding." in: Molecular and cellular biology, Vol. 23, Issue 21, pp. 7488-97, 2003 (PubMed).

Nemoto, Vogt, Oguri et al.: "Activation of the Raf-1/MEK/Erk kinase pathway by a novel Cdc25 inhibitor in human prostate cancer cells." in: The Prostate, Vol. 58, Issue 1, pp. 95-102, 2003 (PubMed).

Ito, Yoshida, Matsuzuka et al.: "Cdc25A and cdc25B expression in malignant lymphoma of the thyroid: correlation with histological subtypes and cell proliferation." in: International journal of molecular medicine, Vol. 13, Issue 3, pp. 431-5, 2004 (PubMed).

Solier, Sordet, Kohn et al.: "Death receptor-induced activation of the Chk2- and histone H2AX-associated DNA damage response pathways." in: Molecular and cellular biology, Vol. 29, Issue 1, pp. 68-82, 2008 (PubMed).

Validation Images
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