You are viewing an incomplete version of our website. Please click to reload the website as full version.

CHEK1 antibody (Checkpoint Kinase 1) (pSer317)

Details for Product anti-CHEK1 Antibody No. ABIN358109, Supplier: Login to see New
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Synonyms An08g10320, AO090003000441, CHK1, chk1, C85740, Chk1, rad27, id:ibd2720, zgc:56093, CHEK1
(55), (53), (37), (33), (29), (20), (15), (13), (13), (10), (9), (7), (5), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
(451), (139), (130), (4), (4), (3), (3), (2), (2), (2), (1), (1)
(393), (49), (23), (6)
This CHEK1 antibody is un-conjugated
(12), (12), (11), (8), (8), (8), (5), (3), (3), (3), (3), (3), (3), (3), (3)
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(370), (173), (125), (59), (54), (36), (25), (22), (22), (7), (5), (4), (2), (1)
Pubmed 5 references available
Supplier Login to see New
Catalog number from supplier Login to see New
Quantity 0.4 mL
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S317 of human CHK1.
Isotype Ig
Specificity This antibody detects CHK1 pSer317.
Purification Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name CHK1 (CHEK1 Antibody Abstract)
Background Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. The S. pombe Chk1 gene encodes a protein kinase that is required for the DNA damage checkpoint. Antibodies against CHK1 recognized a 54-kD protein on immunoblots of mammalian cell extracts. However,CHK1 is modified in response to DNA damage. In vitro, CHK1 directly phosphorylated a regulator of CDC2 tyrosine phosphorylation, CDC25C. In response to DNA damage, CHK1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-cyclin B complex and mitotic entry. CHK1 directly phosphorylates CDC25A during an unperturbed cell cycle, and that phosphorylation of CDC25A by CHK1 is required for cells to delay cell cycle progression in response to double-strand DNA breaks.Synonyms: CHEK-1, CHEK1, CHK1 checkpoint homolog, Serine/threonine-protein kinase Chk1
Molecular Weight 54420 Da
Gene ID 1111, 9606
UniProt O14757
Research Area Kinases/Phosphatases, Cell Cycle
Pathways p53 Signaling, Apoptosis, Cell Division Cycle
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Dot Blot: 1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-CHEK1 antibody (Checkpoint Kinase 1) (pSer317) (ABIN358109) anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody
 image for anti-CHEK1 antibody (Checkpoint Kinase 1) (pSer317) (ABIN358109) anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody (Image 2)
Background publications Krämer, Mailand, Lukas et al.: "Centrosome-associated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase." in: Nature cell biology, Vol. 6, Issue 9, pp. 884-91, 2004 (PubMed).

Xu, Lee, Stern: "Microcephalin is a DNA damage response protein involved in regulation of CHK1 and BRCA1." in: The Journal of biological chemistry, Vol. 279, Issue 33, pp. 34091-4, 2004 (PubMed).

Ng, Lee, Ho et al.: "Differential mode of regulation of the checkpoint kinases CHK1 and CHK2 by their regulatory domains." in: The Journal of biological chemistry, Vol. 279, Issue 10, pp. 8808-19, 2004 (PubMed).

Chen, Ryan, Piwnica-Worms: "Chk1 kinase negatively regulates mitotic function of Cdc25A phosphatase through 14-3-3 binding." in: Molecular and cellular biology, Vol. 23, Issue 21, pp. 7488-97, 2003 (PubMed).

Groth, Lukas, Nigg et al.: "Human Tousled like kinases are targeted by an ATM- and Chk1-dependent DNA damage checkpoint." in: The EMBO journal, Vol. 22, Issue 7, pp. 1676-87, 2003 (PubMed).

Catalog No. ABIN358109
Plus shipping costs $45.00

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)