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Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4) (pSer801) antibody

Details for Product No. ABIN358379, Supplier: Login to see
Antigen
  • FLH21957
  • HGK
  • MEKKK4
  • NIK
  • 9430080K19Rik
  • AU043147
  • AU045934
  • AW046177
  • Nik
Epitope
pSer801
31
20
15
15
13
10
10
7
7
6
6
6
6
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
147
44
25
2
2
2
1
1
1
Host
Rabbit
125
30
Clonality
Polyclonal
Conjugate
Un-conjugated
8
8
8
7
7
7
4
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA)
94
84
36
18
16
14
10
5
4
2
2
1
Supplier
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S801 of human MAP4K4.
Isotype Ig
Specificity This antibody detects MAP4K4 pSer801.
Purification Affinity purification in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name MAP4K4 (MAP4K4 Antibody Abstract)
Background M4K4, a member of the STE20 subfamily of Ser/Thr protein kinases, may play a role in the response to environmental stress and cytokines such as TNF-alpha. It appears to act upstream of the JUN N-terminal pathway. This protein is thought to interact with the SH3 domain of the adapter proteins Nck. HGK binds, via its CNH regulatory domain, to the N-terminal region of SPG3A. Expression appears to be ubiquitous, expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain, and isoform 4 is predominant in the liver, skeletal muscle and placenta.Synonyms: HPK/GCK-like kinase HGK, MAPK/ERK kinase kinase kinase 4, MEKKK 4, Mitogen-activated protein kinase kinase kinase kinase 4, NIK, Nck-interacting kinase
Molecular Weight 142100 Da
Gene ID 9448, 9606
UniProt O95819
Research Area Signaling
Pathways
Application Notes ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4) (pSer801) antibody (ABIN358379) anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4) (pSer801) antibody
Background publications Wright, Wang, Manning et al.: "The STE20 kinase HGK is broadly expressed in human tumor cells and can modulate cellular transformation, invasion, and adhesion." in: Molecular and cellular biology, Vol. 23, Issue 6, pp. 2068-82, 2003 (PubMed).

Yao, Zhou, Wang et al.: "A novel human STE20-related protein kinase, HGK, that specifically activates the c-Jun N-terminal kinase signaling pathway." in: The Journal of biological chemistry, Vol. 274, Issue 4, pp. 2118-25, 1999 (PubMed).

Ishikawa, Nagase, Suyama et al.: "Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 5, Issue 3, pp. 169-76, 1998 (PubMed).