EIF4E antibody (pSer209)

Catalog No. ABIN358406

Product Details anti-EIF4E Antibody

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Binding Specificity: pSer209

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4E antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Specificity: This antibody detectsEIF4E pSer209.

Purification: Affinity purification in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S209 of human EIF4E.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for EIF4E

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Alternative Name: EIF4E (EIF4E Products)

Synonyms: AUTS19 antibody, CBP antibody, EIF4E1 antibody, EIF4EL1 antibody, EIF4F antibody, eif4e-1 antibody, zgc:86680 antibody, EG668879 antibody, Eif4e-ps antibody, If4e antibody, eIF-4E antibody, 0260/09 antibody, 0587/11 antibody, 0589/11 antibody, 0919/12 antibody, 1004/13 antibody, 2 antibody, 7238 antibody, CG4035 antibody, CT13384 antibody, CT39424 antibody, CT39426 antibody, D-eIF4E antibody, Dmel\\CG4035 antibody, Eif4E antibody, Eif4e antibody, IF4E antibody, dEif4e antibody, deIF-4E antibody, deIF4E antibody, eIF 4E antibody, eIF-4E1 antibody, eIF-4E2 antibody, eIF-4EII antibody, eIF-4e antibody, eIF4E antibody, eIF4E-1 antibody, eIF4E-1/2 antibody, eIF4E-2 antibody, eIF4E1 antibody, eIF4EI antibody, eIF4e antibody, eif-4E antibody, eif-4e antibody, eif4e antibody, l(3)07238 antibody, l(3)67Af antibody, l(3)S025007 antibody, l(3)S026009 antibody, l(3)S058711 antibody, l(3)S091912 antibody, cbp antibody, eif4f antibody, eif4e1 antibody, eif4el1 antibody, EIF-4E antibody, zgc:101581 antibody, eukaryotic translation initiation factor 4E antibody, eukaryotic translation initiation factor 4ea antibody, Eukaryotic initiation factor 4E antibody, eucaryotic initiation factor 6 antibody, eukaryotic translation initiation factor 4E L homeolog antibody, eukaryotic translation initiation factor 4E family member 1B S homeolog antibody, translation initiation factor eIF4E antibody, eukaryotic translation initiation factor 4eb antibody, EIF4E antibody, eif4ea antibody, Eif4e antibody, eIF-4E antibody, eif6 antibody, eif4e antibody, eif4e.L antibody, eif4e1b.S antibody, LOC100533326 antibody, eif4eb antibody

Background: EIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Nonphosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E, insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs.Synonyms: EIF-4E, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein

Molecular Weight: 25097 Da

Gene ID: 1977, 9606

UniProt: P06730

Pathways: BCR Signaling