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tyrosine Kinase, Non-Receptor, 2 (TNK2) (pTyr518) antibody

Details for Product No. ABIN358513, Supplier: Log in to see
Antigen
  • ACK
  • ACK-1
  • ACK1
  • p21cdc42Hs
  • AW552477
  • Ack
  • Cdgip
  • Pyk1
  • tyrosine kinase, non-receptor, 2
  • TNK2
  • Tnk2
  • LOC100350340
Epitope
pTyr518
33
18
16
16
15
14
12
12
9
8
8
7
7
6
6
5
5
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
183
79
63
19
4
3
3
Host
Rabbit
182
21
1
Clonality
Polyclonal
Conjugate
Un-conjugated
13
11
11
10
8
8
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA)
136
117
43
36
26
24
19
18
8
5
3
3
2
1
Options
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y518 of human ACK1.
Isotype Ig
Specificity This antibody detects TNK2 / ACK1 pTyr518.
Purification Protein A Chromatography followed by two-step phosphospecific peptide affinity purification.
Alternative Name TNK2 / ACK1 (TNK2 Antibody Abstract)
Background ACK1 is a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway.Synonyms: Activated CDC42 kinase 1
Molecular Weight 114327 Da
Gene ID 10188, 9606
UniProt Q07912
Application Notes ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-tyrosine Kinase, Non-Receptor, 2 (TNK2) (pTyr518) antibody (ABIN358513) anti-tyrosine Kinase, Non-Receptor, 2 (TNK2) (pTyr518) antibody
Background publications Wolf-Yadlin, Hautaniemi, Lauffenburger, White: "Multiple reaction monitoring for robust quantitative proteomic analysis of cellular signaling networks." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 14, pp. 5860-5, 2007 (PubMed).

Cohen: "Varicella-zoster vaccine virus: evolution in action." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 1, pp. 7-8, 2007 (PubMed).

Rikova, Guo, Zeng, Possemato, Yu, Haack, Nardone, Lee, Reeves, Li, Hu, Tan, Stokes, Sullivan, Mitchell, Wetzel, Macneill, Ren, Yuan, Bakalarski, Villen, Kornhauser, Smith, Li, Zhou, Gygi, Gu et al.: "Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer. ..." in: Cell, Vol. 131, Issue 6, pp. 1190-203, 2007 (PubMed).

Goss, Lee, Moritz, Nardone, Spek, MacNeill, Rush, Comb, Polakiewicz: "A common phosphotyrosine signature for the Bcr-Abl kinase." in: Blood, Vol. 107, Issue 12, pp. 4888-97, 2006 (PubMed).