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Antibodies
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anti-Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 2 (ACE2) (N-Term) an...
Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 2 (ACE2) (N-Term) antibody
| Antigen |
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| Synonyms |
ACEH, DKFZp434A014, 2010305L05Rik, ACE2, zgc:92514, MGC127593, DKFZp469K2021, Ace2 |
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Epitope
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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| Catalog no. |
ABIN358579 |
| Quantity |
0.1 mg (0.25 mg/ml) |
| Price |
285.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, Angiotensin-convertingenzyme homolog
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Alternative name
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ACE2
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Gene ID
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59272
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Swiss-Prot
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Q9BYF1
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Immunogen
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This antibody is generated from rabbits immunized with a KLH conjugated syntheticpeptide selected from the N-terminal region of human ACE2.
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Cross-Reactivity
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Human
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Isotype
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Ig (Matching secondary antibodies)
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Description
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ACE2 cDNA encodes a deduced 805-amino acid protein containing a potential 17-aminoacid N-terminal signal peptide and a putative 22-amino acid C-terminal membrane anchor. It also possesses a zinc metalloprotease consensus sequence and a conserved glutamineresidue that may function as a third zinc ligand. ACE2 is expressed predominantly invascular endothelial cells of the heart and kidney. ACE converts angiotensin I toangiotensin II, ACE2 converts angiotensin I to angiotensin 1-9, which has 9 amino acids. Angiotensin II is a potent blood vessel constrictor, while angiotensin 1-9 does not impactblood vessels but is cleaved by ACE to a shorter peptide, angiotensin 1-7, which is a bloodvessel dilator. Spike (S) proteins of coronaviruses, including the SARS coronavirus, bindwith cellular receptors to mediate infection of target cells. ACE2 binds the S1 domain of theSARS coronavirus S protein. SARS coronavirus replicates efficiently on ACE2-transfectedbut not mock-transfected 293T cells. Anti-ACE2 but not anti-ACE1 antibody blocks viralreplication on Vero E6 cells. It has been proposed that ACE2 is a functional receptor forSARS coronavirus.
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Specificity
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This antibody reacts to ACE2 (SARS Receptor). Species: Human. Others not tested.
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Application Notes
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ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/10 - 1/50. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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0.25 mg/ml
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Purification
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Purified
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Buffer
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PBS with 0.09% (W/V) sodium azide
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Storage
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Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Research Area
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Metabolism, Enzymes, Serum/Plasma Proteins
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Restrictions
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For Research Use only
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Alternatives for antigen "Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 2 (ACE2)", type "Antibodies"
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Hosts
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Rabbit (57), Mouse (1)
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Reactivities
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Human (57), Mouse (Murine) (24), Rat (Rattus) (20), Dog (Canine) (16), Pig (Porcine) (16), Sheep (Ovine) (15), Cow (Bovine) (1), Horse (Equine) (1)
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Applications
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Western Blotting (WB) (40), Flow Cytometry (FACS) (20), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (20), Immunofluorescence (IF) (18), ELISA (13), Enzyme Immunoassay (EIA) (12), Immunohistochemistry (IHC) (8), Immunoprecipitation (IP) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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C-Term (3), N-Term (3), Center (2), Middle (1), N-Term K187 (1)
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