CRY1 antibody (C-Term)

Catalog No. ABIN358613

Product Details anti-CRY1 Antibody

Target: CRY1 (Cryptochrome 1 (Photolyase-Like) (CRY1))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRY1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to CRY1.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide C-terminal region of human Cry1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for CRY1

Target: CRY1 (Cryptochrome 1 (Photolyase-Like) (CRY1))

Alternative Name: Cryptochrome-1 (CRY1 Products)

Synonyms: cry1-A antibody, CRY1 antibody, cry2 antibody, phll1 antibody, xCRY1 antibody, PHLL1 antibody, AU020726 antibody, AU021000 antibody, Phll1 antibody, ATCRY1 antibody, BLU1 antibody, BLUE LIGHT UNINHIBITED 1 antibody, CRYPTOCHROME 1 APOPROTEIN (BLUE LIGHT PHOTORECEPTOR antibody, ELONGATED HYPOCOTYL 4 antibody, HY4 antibody, OOP2 antibody, OUT OF PHASE 2 antibody, T3H13.14 antibody, T3H13_14 antibody, cryptochrome 1 antibody, cryptochrome circadian clock 1 L homeolog antibody, cryptochrome circadian regulator 1 antibody, cryptochrome circadian clock 1 antibody, Cryptochrome-1 antibody, cryptochrome 1 antibody, cryptochrome 1 (photolyase-like) antibody, cry1.L antibody, CRY1 antibody, cry1 antibody, siu50817b antibody, Cry1 antibody

Background: Various biochemical, physiological and behavioural processes display circadian rhythms controlled by an internal biological clock. The central gears driving this clock appear to be composed of an autoregulatory transcription/posttranslation-based feedback loop. Cryptochrome 1 (CRY1) and 2 (CRY2) are DNA-binding flavoproteins that bear some homology to blue-light receptors and photolyases. In Drosophila, CRY is a photoreceptor for the circadian clock where it binds to the clock component TIM in a light-dependent fashion and blocks its function. Mammalian CRY1 and CRY2 function via light-independent interactions with circadian genes CLOCK and BMAL1, as well as with PER1, PER2, and TIM. They seem to act as light-independent components of the circadian clock and likely regulate Per1 transcriptional cycling via interactions with both the activator and its feedback inhibitors. Mutant mice not expressing the Cry1 or Cry2 protein display accelerated and delayed periodicity of locomotor activity, respectively. It appears that the combination of both proteins working together is essential to synchronize the organism to circadian phases. A critical balance between Cry1 and Cry2 is required for proper clock function, in complete darkness, double-mutant mice present with instantaneous arrhythmicity, indicating the absence of an internal circadian clock.Synonyms: CRY1, PHLL1

Gene ID: 1407, 9606

UniProt: Q16526

Pathways: Response to Water Deprivation, Proton Transport