Myeloid/lymphoid Or Mixed-Lineage Leukemia (Trithorax Homolog, Drosophila), Translocated To, 3 (MLLT3) (N-Term) antibody

Details for Product No. ABIN358675, Supplier: Log in to see
Antigen
  • fc39c11
  • wu:fc39c11
  • zgc:110210
  • AF9
  • YEATS3
  • 2210011H10Rik
  • 2610012I03Rik
  • 3830408D16Rik
  • Af9
  • D4Ertd321e
  • Af-9
  • myeloid/lymphoid or mixed lineage-leukemia translocation to 3 homolog (Drosophila)
  • myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3
  • mllt3
  • MLLT3
  • Mllt3
Epitope
N-Term
32
14
9
8
6
5
4
4
4
4
3
2
2
1
1
1
1
1
Reactivity
Human
83
19
10
9
8
8
7
6
5
3
3
2
2
1
Host
Rabbit
80
3
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
71
38
31
29
21
17
7
3
2
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Immunogen KLH conjugated synthetic peptide selected from the N-terminal region of Human MLLT3
Isotype Ig
Specificity This antibody recognizes Human MLLT3 (N-term).
Purification Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
Alternative Name MLLT3 / AF9 (MLLT3 Antibody Abstract)
Background The human AF9 gene is one of the most common fusion partner genes with the ALL1 gene at 11q23 (also called MLL), resulting in the t(9,11)(p22,q23). The AF9 gene is more than 100 kb, and 2 patient breakpoint cluster regions (BCRs) have been identified, BCR1 is within intron 4, previously called site A, whereas BCR2 or site B spans introns 7 and 8. Several different structural elements have been identified in AF9, including a colocalizing in vivo DNA topo II cleavage site and an in vitro DNase I hypersensitive (DNase 1 HS) site in intron 7 in BCR2. Reversibility experiments demonstrated a religation of the topo II cleavage sites. In addition, 2 scaffold associated regions (SARs) are located centromeric to the topo II and DNase I HS cleavage sites and border breakpoint regions in 2 leukemic cells lines: SAR1 is located in intron 4, whereas SAR2 encompasses parts of exons 5-7. The patient breakpoint regions of AF9 share the same structural elements as the MLL BCR. A DNA breakage and repair model for nonhomologous recombination between MLL and its partner genes, particularly AF9, has been proposed.Synonyms: ALL1 fused gene from chromosome 9 protein, Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein, Protein AF-9, YEATS domain-containing protein 3, YEATS3
Gene ID 4300, 9606
UniProt P42568
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-Myeloid/lymphoid Or Mixed-Lineage Leukemia (Trithorax Homolog, Drosophila), Translocated To, 3 (MLLT3) (N-Term) antibody (ABIN358675) anti-Myeloid/lymphoid Or Mixed-Lineage Leukemia (Trithorax Homolog, Drosophila), Translocated To, 3 (MLLT3) (N-Term) antibody
Background publications Strissel, Strick, Tomek, Roe, Rowley, Zeleznik-Le: "DNA structural properties of AF9 are similar to MLL and could act as recombination hot spots resulting in MLL/AF9 translocations and leukemogenesis." in: Human molecular genetics, Vol. 9, Issue 11, pp. 1671-9, 2000 (PubMed).

Iida, Seto, Yamamoto, Komatsu, Tojo, Asano, Kamada, Ariyoshi, Takahashi, Ueda: "MLLT3 gene on 9p22 involved in t(9;11) leukemia encodes a serine/proline rich protein homologous to MLLT1 on 19p13." in: Oncogene, Vol. 8, Issue 11, pp. 3085-92, 1993 (PubMed).

Nakamura, Alder, Gu, Prasad, Canaani, Kamada, Gale, Lange, Crist, Nowell: "Genes on chromosomes 4, 9, and 19 involved in 11q23 abnormalities in acute leukemia share sequence homology and/or common motifs." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 10, pp. 4631-5, 1993 (PubMed).

Did you look for something else?