MTOR antibody (Ser2481)

Catalog No. ABIN358774

Product Details anti-MTOR Antibody

Target: MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

Binding Specificity: Ser2481

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MTOR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to mTOR (FRAP1).

Purification: Protein A column, followed by peptide affinity purification

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S2481 of human FRAP1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for MTOR

Target: MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

Alternative Name: mTOR / FRAP1 (mTOR Products)

Synonyms: FRAP1 antibody, FRAP antibody, FRAP2 antibody, RAFT1 antibody, RAPT1 antibody, 2610315D21Rik antibody, AI327068 antibody, Frap1 antibody, flat antibody, frap1 antibody, tor antibody, wu:fc22h08 antibody, mechanistic target of rapamycin kinase antibody, mechanistic target of rapamycin antibody, mechanistic target of rapamycin (serine/threonine kinase) antibody, MTOR antibody, Mtor antibody, mtor antibody

Background: FRAP1 belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. FRAP1 is a part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.Synonyms: FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FRAP, FRAP2, Mammalian target of rapamycin, RAPT1, Rapamycin target protein, Serine/threonine-protein kinase mTOR, TOR

Gene ID: 2475, 9606

UniProt: P42345

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, BCR Signaling, Warburg Effect