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PRKAA1 antibody (Ser487)

Name: PRKAA1 antibody (Ser487)
SKU: ABIN359481
Price: 744 USD
Availability: InStock

PRKAA1 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal unconjugated

Western blot analysis of PRKAA1 (arrow) using rabbit polyclonal PRKAA1 Antibody .

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with PRKAA1, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

IHC (p)

2 images

Catalog No. ABIN359481

Datasheet as PDF

Target See all PRKAA1 Antibodies

PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))
Binding Specificity
30

17

16

15

8

7

7

7

6

5

4

4

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Ser487
Reactivity
155

132

89

27

11

9

9

8

7

7

5

5

4

3

2

2

2

1

1

1

1
Human
Host
155

17

1
Rabbit
Compatible Secondaries
Clonality
143

30
Polyclonal
Conjugate
85

10

9

9

5

5

5

5

5

5

5

4

4

4

4

4

3

1

1
This PRKAA1 antibody is un-conjugated
Application
136

58

45

41

40

39

34

20

13

12

11

3

2

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity

This antibody detects AMPK alpha (PRKAA1).

Purification

Protein A column, followed by peptide affinity purification.

Immunogen

This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S487 of human PRKAA1.

Isotype

Ig Fraction

Top Product

Discover our top product PRKAA1 Primary Antibody
anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (AA 1-300) antibody
PRKAA1 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (AA 28-257) antibody
PRKAA1 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) antibody
PRKAA1 Reactivity: Human, Mouse, Rat, Monkey WB, IHC, ELISA, FACS, ICC Host: Mouse Monoclonal 2B7 unconjugated

anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (pThr172), (pThr183) antibody
PRKAA1 Reactivity: Human, Mouse, Rat WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (AA 351-450) antibody
PRKAA1 Reactivity: Human, Mouse, Rat, Pig, Sheep WB, IHC (p), FACS, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (AA 351-450) antibody
PRKAA1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), FACS Host: Rabbit Polyclonal unconjugated

anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (AA 451-550) antibody
PRKAA1 Reactivity: Human WB, ELISA, IHC (p), IF Host: Mouse Monoclonal 4D8 unconjugated

anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (AA 325-543), (Catalytic Subunit alpha) antibody
PRKAA1 Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (pSer486) antibody
PRKAA1 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (AA 451-550) antibody
PRKAA1 Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 4D9 unconjugated

Application Notes

ELISA 1: 1,000. Western blot 1: 50 - 1: 100. Immunohistochemistry 1: 10 - 1: 50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Format

Liquid

Concentration

0.25 mg/mL

Buffer

PBS with 0.09 % (W/V) sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing.

Storage

4 °C/-20 °C

Storage Comment

Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
Target

PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Alternative Name

PRKAA1 (PRKAA1 Products)

Synonyms

AMPK antibody, AMPKa1 antibody, cb116 antibody, im:7154392 antibody, sb:cb130 antibody, wu:fa94c10 antibody, AMPK1 antibody, AMPKalpha1 antibody, AI194361 antibody, AI450832 antibody, AL024255 antibody, C130083N04Rik antibody, ampk antibody, ampka1 antibody, PRKAA1 antibody, protein kinase AMP-activated catalytic subunit alpha 1 antibody, protein kinase, AMP-activated, alpha 1 catalytic subunit antibody, protein kinase, AMP-activated, alpha 1 catalytic subunit L homeolog antibody, PRKAA1 antibody, prkaa1 antibody, Prkaa1 antibody, prkaa1.L antibody

Background

PRKAA1 belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.Synonyms: 5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK alpha-1 chain, AMPK1

Molecular Weight

63878 Da

Gene ID

5562, 9606

UniProt

Q13131

Pathways

AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect