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DDX5 antibody (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5) (C-Term)

Details for Product anti-DDX5 Antibody No. ABIN359623, Supplier: Log in to see
Antigen
  • 2600009A06Rik
  • G17P1
  • Hlr1
  • HLR1
  • HUMP68
  • p68
  • wu:fa56a07
  • wu:fb11e01
  • wu:fb16c10
  • wu:fb53b05
Epitope
C-Term
37
20
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
106
44
41
11
4
3
2
2
1
1
Host
Rabbit
73
20
13
Clonality
Polyclonal
Conjugate
This DDX5 antibody is un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
76
39
34
31
20
12
10
9
4
3
3
1
1
Supplier
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human DDX5.
Isotype Ig
Specificity This antibody reacts to DDX5.
Purification Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
Alternative Name DDX5 (DDX5 Antibody Abstract)
Background DDX5 is putative RNA helicases. The protein is implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This protein is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen.Synonyms: DDX-5, DEAD box protein 5, G17P1, HELR, HLR1, Probable ATP-dependent RNA helicase DDX5, RNA helicase p68
Gene ID 1655, 9606
UniProt P17844
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-DDX5 antibody (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5) (C-Term) (ABIN359623) Western blot analysis of DDX5 antibody (C-term) in T47D cell line lysates (35ug/lane)...
Product cited in: Daub, Olsen, Bairlein, Gnad, Oppermann, Körner, Greff, Kéri, Stemmann, Mann: "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." in: Molecular cell, Vol. 31, Issue 3, pp. 438-48, 2008

Background publications Ong, Mittler, Mann: "Identifying and quantifying in vivo methylation sites by heavy methyl SILAC." in: Nature methods, Vol. 1, Issue 2, pp. 119-26, 2005