CDK8 antibody (N-Term)

Catalog No. ABIN359669

Product Details anti-CDK8 Antibody

Target: CDK8 (Cyclin-Dependent Kinase 8 (CDK8))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK8 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to CDK8.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human CDK8.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for CDK8

Target: CDK8 (Cyclin-Dependent Kinase 8 (CDK8))

Alternative Name: CDK8 (CDK8 Products)

Synonyms: K35 antibody, wu:fk95h11 antibody, cyclin dependent kinase 8 antibody, cyclin-dependent kinase 8 antibody, cyclin-dependent kinase 8 S homeolog antibody, Cyclin-dependent kinase 8 antibody, cyclin dependent kinase 19 antibody, CDK8 antibody, Cdk8 antibody, cdk8 antibody, cdk8.S antibody, cdk-8 antibody, CDK19 antibody

Background: CDK8 is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery.Synonyms: Cell division protein kinase 8, Cyclin-dependent kinase 8, Mediator complex subunit CDK8, Mediator of RNA polymerase II transcription subunit CDK8, Protein kinase K35

Gene ID: 1024, 9606

UniProt: P49336

Pathways: Cell Division Cycle, Regulation of Lipid Metabolism by PPARalpha