CDKN1A antibody (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1)) (C-Term)

Details for Product anti-CDKN1A Antibody No. ABIN359673, Supplier: Log in to see
Antigen
  • p21
  • cip1
  • p16Xic2
  • CCND1
  • sdi1
  • waf1
  • cap20
  • cdkn1
  • mda-6
  • p21cip1
  • CDKN1A
  • si:ch1073-48m5.2
  • Cip1
  • UV96
  • Waf1
  • CAP20
  • CDKN1
  • CIP1
  • MDA-6
  • P21
  • SDI1
  • WAF1
  • p21CIP1
  • CDKI
  • Cdkn1
  • mda6
  • p21Cip1
  • p21WAF
  • cyclin-dependent kinase inhibitor 1A (p21, Cip1)
  • cyclin-dependent kinase inhibitor 1
  • cyclin-dependent kinase inhibitor 1A
  • cyclin-dependent kinase inhibitor 1A (P21)
  • cdkn1a
  • cki1
  • CDKN1A
  • LOC100348489
  • Cdkn1a
Alternatives
anti-Non-Human Primate CDKN1A antibody for Immunocytochemistry
Epitope
C-Term
51
40
38
27
20
20
18
18
18
18
15
15
15
15
12
9
8
7
7
5
4
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
Reactivity
Human
580
184
124
30
23
9
6
5
4
2
1
1
1
1
1
1
1
1
Host
Rabbit
311
310
2
1
Clonality
Polyclonal
Conjugate
This CDKN1A antibody is un-conjugated
33
32
32
23
16
16
15
15
12
12
10
10
10
10
10
10
10
8
8
6
6
6
6
4
4
4
4
4
4
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
356
223
186
167
151
140
67
60
59
53
30
14
6
6
6
2
1
1
Options
Supplier
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Supplier Product No.
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human CDKN1A.
Isotype Ig
Specificity This antibody reacts to p21 (CDKN1A).
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Alternative Name CDKN1A / p21WAF1 (CDKN1A Antibody Abstract)
Background CDKN1A is a potent cyclin-dependent kinase inhibitor. It binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. Expression is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.Synonyms: CAP20, CDK-interacting protein 1, CDKN1, CIP1, Cyclin-dependent kinase inhibitor 1, MDA-6, MDA6, Melanoma differentiation-associated protein 6, PIC1, SDI1, WAF1
Gene ID 1026, 9606
UniProt P38936
Pathways p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (C-Term) antibody (ABIN359673) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Western Blotting (WB) image for anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (C-Term) antibody (ABIN359673) The anti-CDKN1A Pab is used in Western blot to detect CDKN1A in T-47D cell lysate.
Background publications Fukuchi, Nakamura, Ichimura, Tatsumi, Gomi: "The association of cyclin A and cyclin kinase inhibitor p21 in response to gamma-irradiation requires the CDK2 binding region, but not the Cy motif." in: Biochimica et biophysica acta, Vol. 1642, Issue 3, pp. 163-71, 2003 (PubMed).

Frouin, Maga, Denegri, Riva, Savio, Spadari, Prosperi, Scovassi: "Human proliferating cell nuclear antigen, poly(ADP-ribose) polymerase-1, and p21waf1/cip1. A dynamic exchange of partners." in: The Journal of biological chemistry, Vol. 278, Issue 41, pp. 39265-8, 2003 (PubMed).

Dupont, Karas, LeRoith: "The cyclin-dependent kinase inhibitor p21CIP/WAF is a positive regulator of insulin-like growth factor I-induced cell proliferation in MCF-7 human breast cancer cells." in: The Journal of biological chemistry, Vol. 278, Issue 39, pp. 37256-64, 2003 (PubMed).

Di Padova, Bruno, De Nicola, Iezzi, DAngelo, Gallo, Nicosia, Corbi, Biroccio, Floridi, Passananti, Fanciulli: "Che-1 arrests human colon carcinoma cell proliferation by displacing HDAC1 from the p21WAF1/CIP1 promoter." in: The Journal of biological chemistry, Vol. 278, Issue 38, pp. 36496-504, 2003 (PubMed).

Bai, Miyake, Iwai, Yuasa: "CDX2, a homeobox transcription factor, upregulates transcription of the p21/WAF1/CIP1 gene." in: Oncogene, Vol. 22, Issue 39, pp. 7942-9, 2003 (PubMed).

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