ITK antibody (Middle Region)

Catalog No. ABIN359985

Product Details anti-ITK Antibody

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Binding Specificity: Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITK antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to ITK.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human ITK.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for ITK

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Alternative Name: Tyrosine-Protein Kinase ITK/TSK (ITK Products)

Synonyms: Emt antibody, Tcsk antibody, Tsk antibody, EMT antibody, LPFS1 antibody, LYK antibody, PSCTK2 antibody, IL2 inducible T cell kinase antibody, IL2 inducible T-cell kinase antibody, IL2-inducible T-cell kinase antibody, Itk antibody, ITK antibody

Background: ITK is an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation.Synonyms: EMT, Kinase EMT, LYK, T-cell-specific kinase, Tyrosine-protein kinase Lyk

Gene ID: 3702

NCBI Accession: NP_005537

UniProt: Q08881

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway