TRIB1 antibody (C-Term)

Catalog No. ABIN360003

Product Details anti-TRIB1 Antibody

Target: TRIB1 (Tribbles Homolog 1 (TRIB1))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIB1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to TRIB1.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human TRIB1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for TRIB1

Target: TRIB1 (Tribbles Homolog 1 (TRIB1))

Alternative Name: TRIB1 (TRIB1 Products)

Synonyms: TRIB1 antibody, c8fw antibody, gig2 antibody, skip1 antibody, trb1 antibody, tribbles antibody, C8FW antibody, GIG2 antibody, SKIP1 antibody, TRB1 antibody, A530090O15Rik antibody, TRB-1 antibody, Trb1 antibody, Gig2 antibody, tribbles pseudokinase 1 antibody, tribbles pseudokinase 1 L homeolog antibody, tribbles homolog 1 (Drosophila) antibody, TRIB1 antibody, trib1 antibody, trib1.L antibody, Trib1 antibody

Background: TRIB1 antibody, also called gprotein coupled receptor induced, skip1, gig2, c8fwATP, functions in binding protein binding, protein kinase inhibitor activity, and protein serine/threonine kinase activities. Overexpression of TRIB1 in HeLa cells repressed the basal activity of the IL8 promoter by inhibiting AP1 activity. Overexpression of TRIB1 inhibited oncogenic Ras -driven AP1 activation and MEKK1-mediated AP1 activation. ERK activation was enhanced by TRIB1. Coimmunoprecipitation and yeast 2-hybrid assays showed that MEK1 interacted with both TRIB1 and TRIB3, and MKK4 interacted specifically with TRIB1. Cotransfection of MKK4 enhanced the level of TRIB1, indicating that the TRIB-MAPKK interaction stabilized TRIB1.Synonyms: C8FW, GIG-2, GIG2, SKIP1, TRB-1, TRB1, Tribbles homolog 1

Gene ID: 10221, 9606

UniProt: Q96RU8

Pathways: Smooth Muscle Cell Migration