PUM2 antibody (Pumilio Homolog 2 (Drosophila)) (Ser182)

Details for Product anti-PUM2 Antibody No. ABIN360017, Supplier: Log in to see
Antigen
  • pum-2
  • 5730503J23Rik
  • Pumm2
  • mKIAA0235
  • PUMH2
  • PUML2
  • pumilio-2
  • pumilio 2 (Drosophila)
  • pumilio homolog 2 (Drosophila)
  • pumilio RNA-binding family member 2
  • pum-2
  • Pum2
  • PUM2
Alternatives
anti-Human PUM2 antibody for ELISA
Epitope
Ser182
23
16
11
8
7
4
3
3
2
2
1
1
1
1
1
1
Reactivity
Human
108
35
28
8
7
6
6
5
3
3
1
1
1
1
Host
Rabbit
77
16
15
1
Clonality
Polyclonal
Conjugate
This PUM2 antibody is un-conjugated
6
6
6
5
5
5
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
91
60
31
13
12
8
3
3
2
1
1
Options
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Ser182 of Human PUM2.
Isotype Ig
Specificity This antibody recognizes Pumilio 2 (PUM2).
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Protein A Chromatography followed by peptide affinity purification.
Alternative Name Pumilio 2 / PUM2 (PUM2 Antibody Abstract)
Background PUM2 is a sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.Synonyms: KIAA0235, PUM-2, PUMH2, Pumilio homolog 2, Pumilio-2
Molecular Weight 114216 Da
Gene ID 23369, 9606
UniProt Q8TB72
Pathways Ribonucleoprotein Complex Subunit Organization
Application Notes ELISA: 1/1,000. Western blot 1/50approx. 1/100. Immunohistochemistry: 1/10approx. 1/50. Flow Cytometry: 1/10approx. 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-PUM2 antibody (Pumilio Homolog 2 (Drosophila)) (Ser182) (ABIN360017) anti-Pumilio Homolog 2 (Drosophila) (PUM2) (Ser182) antibody
 image for anti-PUM2 antibody (Pumilio Homolog 2 (Drosophila)) (Ser182) (ABIN360017) anti-Pumilio Homolog 2 (Drosophila) (PUM2) (Ser182) antibody (Image 2)
 image for anti-PUM2 antibody (Pumilio Homolog 2 (Drosophila)) (Ser182) (ABIN360017) anti-Pumilio Homolog 2 (Drosophila) (PUM2) (Ser182) antibody (Image 3)
Background publications Kusz, Ginter-Matuszewska, Ziolkowska, Spik, Bierla, Jedrzejczak, Latos-Bielenska, Pawelczyk, Jaruzelska: "Polymorphisms of the human PUMILIO2 gene and male sterility." in: Molecular reproduction and development, Vol. 74, Issue 6, pp. 795-9, 2007 (PubMed).

Spik, Oczkowski, Olszak, Kotecki, Formanowicz, B?azewicz, Jaruzelska: "Candidate mRNAs interacting with fertility protein PUMILIO2 in the human germ line." in: Reproductive biology, Vol. 6 Suppl 1, pp. 37-42, 2006 (PubMed).

Moore, Jaruzelska, Fox, Urano, Firpo, Turek, Dorfman, Pera: "Human Pumilio-2 is expressed in embryonic stem cells and germ cells and interacts with DAZ (Deleted in AZoospermia) and DAZ-like proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 538-43, 2003 (PubMed).