STK39 antibody (Middle Region)

Catalog No. ABIN360385

Product Details anti-STK39 Antibody

Target: STK39 (serine threonine Kinase 39 (STK39))

Binding Specificity: Middle Region

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STK39 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to SPAK.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the central region of human SPAK.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for STK39

Target: STK39 (serine threonine Kinase 39 (STK39))

Alternative Name: STK39 / SPAK (STK39 Products)

Synonyms: dcht antibody, pask antibody, spak antibody, MGC69537 antibody, DCHT antibody, PASK antibody, SPAK antibody, Spak antibody, AW227544 antibody, AW556857 antibody, RF005 antibody, Rnl5 antibody, serine/threonine kinase 39 antibody, serine/threonine kinase 39 L homeolog antibody, serine threonine kinase 39 antibody, stk39 antibody, STK39 antibody, stk39.L antibody, Stk39 antibody

Background: SPAK is a serine/threonine kinase containing an N-terminal series of proline and alanine repeats (PAPA box), followed by a serine/threonine kinase catalytic domain, a nuclear localization signal, a consensus caspase cleavage recognition motif, and a C-terminal region. Northern blot analysis detects ubiquitous expression, most abundantly in brain and pancreas. SPAK can phosphorylate itself and an exogenous substrate in vitro. SPAK immunoprecipitates from transfected mammalian cells in a complex with another serine/threonine kinase that phosphorylates catalytically inactive SPAK. SPAK activates the p38 MAP kinase pathway in cotransfection assays. Full-length SPAK is expressed in the cytoplasm in transfected cells, while a mutant corresponding to caspase-cleaved STK39 localizes predominantly in the nucleus.Synonyms: DCHT, STE20/SPS1-related proline-alanine-rich protein kinase, Serine/threonine-protein kinase 39, Ste-20-related kinase

Gene ID: 27347, 9606

UniProt: Q9UEW8