PIP5K1A antibody (N-Term)

Catalog No. ABIN360478

Product Details anti-PIP5K1A Antibody

Target: PIP5K1A (Phosphatidylinositol-4-Phosphate 5-Kinase, Type I, alpha (PIP5K1A))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIP5K1A antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to PIP5K1A.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PIP5K1A.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PIP5K1A

Target: PIP5K1A (Phosphatidylinositol-4-Phosphate 5-Kinase, Type I, alpha (PIP5K1A))

Alternative Name: PIP5K1A (PIP5K1A Products)

Synonyms: Pip5k1b antibody, pip5k1c antibody, PIP5K1A antibody, pip5k1b antibody, Pipk5a antibody, Pipk5b antibody, pip5k1a antibody, zgc:110576 antibody, im:7140933 antibody, zgc:158838 antibody, phosphatidylinositol-4-phosphate 5-kinase type 1 alpha antibody, phosphatidylinositol-4-phosphate 5-kinase, type 1, alpha antibody, phosphatidylinositol-4-phosphate 5-kinase, type I, alpha L homeolog antibody, phosphatidylinositol-4-phosphate 5-kinase, type I, alpha antibody, phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha antibody, phosphatidylinositol-4-phosphate 5-kinase, type I, alpha, a antibody, phosphatidylinositol-4-phosphate 5-kinase, type I, alpha, b antibody, PIP5K1A antibody, Pip5k1a antibody, pip5k1a.L antibody, pip5k1a antibody, pip5k1aa antibody, pip5k1ab antibody

Background: Overexpression of phosphatidylinositol phosphate 5-kinase alpha (PIP5KIalpha), which synthesizes PIP2, suppresses apoptosis, whereas a kinase-deficient mutant does not. Protection by the wild-type PIP5KIalpha isaccompanied by decreases in the generation of activated caspases and of caspase 3-cleaved PARP. Protection is not mediated through PIP3 or Akt activation. An anti-apoptotic role for PIP(2) is substantiated by the finding that PIP5KIalpha is cleaved by caspase 3 during apoptosis, and cleavage inactivates PIP5KIalpha in vitro. Mutation of the P(4) position (D279A) of the PIP5KIalpha caspase 3 cleavage consensus prevents cleavage in vitro, and during apoptosis in vivo. Significantly, the caspase 3-resistant PIP5KIalpha mutant is more effective in suppressing apoptosis than the wild-type kinase. PIP2 is a direct regulator of apical and effector caspases in the death receptor and mitochondrial pathways, and PIP5KIalpha inactivation contributes to the progression of apoptosis.Synonyms: 68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha, PIP5K1-alpha, PIP5KIalpha, Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha, PtdIns(4)P-5-kinase 1 alpha

Gene ID: 8394, 9606

UniProt: Q99755

Pathways: PI3K-Akt Signaling, Mitotic G1-G1/S Phases, Inositol Metabolic Process, DNA Replication, Cell-Cell Junction Organization, Synthesis of DNA