Diacylglycerol Kinase, delta 130kDa (DGKD) (C-Term) antibody

Details for Product No. ABIN360608
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Antigen
Synonyms DGKdelta, dgkd-2, AI841987, D330025K09, RGD1563309
Epitope
C-Term
(4), (2), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(19), (2)
Host
Rabbit
(20)
Clonality
Polyclonal
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(20), (9), (3), (2), (2), (1)
Pubmed 2 references available
Catalog no. ABIN360608
Quantity 0.4 mL
Price
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human DGKD.
Specificity This antibody reacts to DGKD.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Alternative Name DAG kinase delta
Background Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).
Alternate names: DGK-delta, DGKD, Diacylglycerol kinase delta, Diglyceride kinase delta, KIAA0145
Gene ID 8527
NCBI Accession 9606
UniProt Q16760
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09% (w/v) sodium azide
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Supplier Images
anti-Diacylglycerol Kinase, delta 130kDa (DGKD) (C-Term) antibody anti-Diacylglycerol Kinase, delta 130kDa (DGKD) (C-Term) antibody
anti-Diacylglycerol Kinase, delta 130kDa (DGKD) (C-Term) antibody (2) anti-Diacylglycerol Kinase, delta 130kDa (DGKD) (C-Term) antibody (Image 2)
Background publications Nagase, Seki, Tanaka et al.: "Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 2, Issue 4, pp. 167-74, 199-210, 1996 (PubMed).

Sakane, Imai, Kai et al.: "Molecular cloning of a novel diacylglycerol kinase isozyme with a pleckstrin homology domain and a C-terminal tail similar to those of the EPH family of protein-tyrosine kinases." in: The Journal of biological chemistry, Vol. 271, Issue 14, pp. 8394-401, 1996 (PubMed).

Hosts (20)
Reactivities (19), (2)
Applications (20), (9), (3), (2), (2), (1)
Epitopes (4), (2), (1), (1), (1), (1), (1)
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