BRSK1 antibody (N-Term)

Catalog No. ABIN360694

Product Details anti-BRSK1 Antibody

Target: BRSK1 (BR Serine/threonine Kinase 1 (BRSK1))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRSK1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to BRSK1.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human BRSK1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for BRSK1

Target: BRSK1 (BR Serine/threonine Kinase 1 (BRSK1))

Alternative Name: BRSK1 (BRSK1 Products)

Synonyms: BRSK1 antibody, hSAD1 antibody, Gm1100 antibody, SAD-B antibody, SADB antibody, RGD1563268 antibody, Sad-b antibody, BR serine/threonine kinase 1 antibody, BRSK1 antibody, Brsk1 antibody

Background: BRSK1 may be involved as a checkpoint kinase in the regulation of G2/M arrest in response to UV- or methyl methane sulfonate (MMS)-induced, but not IR-induced, DNA damage. This protein phosphorylates WEE1 and CDC25B in vitro and CDC25C in vitro and in vivo. BRSK1 is partitioned between cytoplasmic and nuclear locations in the absence of DNA damage, but translocates to the nucleus in response to Uv- or MMS-induced DNA damage. BRSK1 shares significant homology with the fission yeast Cdr2, a mitosis-regulatory kinase, and Caenorhabditis elegans SAD1, a neuronal cell polarity regulator. The BRSK1 transcript is expressed ubiquitously with the highest levels of expression in brain and testis.Synonyms: BR serine/threonine-protein kinase 1, KIAA1811, SAD-B, SAD1, SAD1 kinase, Serine/threonine kinase SAD-B

Gene ID: 84446, 9606

UniProt: Q8TDC3