Lamin B1 antibody (LMNB1)

Details for Product anti-LMNB1 Antibody No. ABIN360850, Supplier: Log in to see
Antigen
  • MGC52550
  • Lamin-L(I)
  • lamin-b
  • lmn
  • lmn2
  • lmnb
  • LMNB1
  • ADLD
  • LMN
  • LMN2
  • LMNB
  • fc06g01
  • wu:fc06g01
  • lamin B1
  • lmnb1
  • LMNB1
  • LOC100037680
  • Lmnb1
Alternatives
anti-Mouse (Murine) Lamin B1 antibody for Western Blotting
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine), Zebrafish (Danio rerio)
148
69
60
15
13
13
8
5
4
4
4
3
3
2
1
1
1
1
Host
Mouse
88
37
28
Clonality (Clone)
Monoclonal ()
Conjugate
This Lamin B1 antibody is un-conjugated
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
143
63
46
30
29
23
15
13
9
4
4
2
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen Purified Rat liver lamins.
Clone 119D5-F1
Isotype IgG1
Specificity The antibody Clone 119D5-F1 reacts with an epitope located C-terminal of residue 231 in Lamin B1.
Purification Purified
Alternative Name Lamin-B1 (LMNB1) (LMNB1 Antibody Abstract)
Background Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane.Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2.Synonyms: LMN2, LMNB, LMNB-1, LMNB1, Lamin-B1, Nuclear Envelope Marker
Molecular Weight 67 kDa (Predicted)
Gene ID 4001
NCBI Accession NP_005564
UniProt P20700
Research Area Cytoskeleton
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes Immunocytochemistry (1: 100 - 1: 200 with avidin-biotinylated horseradish peroxidasecomplex (ABC) as detection reagent). Immunoblotting (1: 100 - 1: 1000). Flow cytometry (1: 100 - 1: 200). ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Broers, Bronnenberg, Kuijpers, Schutte, Hutchison, Ramaekers: "Partial cleavage of A-type lamins concurs with their total disintegration from the nuclear lamina during apoptosis." in: European journal of cell biology, Vol. 81, Issue 12, pp. 677-91, 2003 (PubMed).

Neri, Raymond, Giordano, Capitani, Martelli: "Lamin A is part of the internal nucleoskeleton of human erythroleukemia cells." in: Journal of cellular physiology, Vol. 178, Issue 3, pp. 284-95, 1999 (PubMed).

Broers, Machiels, Kuijpers, Smedts, van den Kieboom, Raymond, Ramaekers: "A- and B-type lamins are differentially expressed in normal human tissues." in: Histochemistry and cell biology, Vol. 107, Issue 6, pp. 505-17, 1997 (PubMed).

Machiels, Ramaekers, Kuijpers, Groenewoud, Oosterhuis, Looijenga: "Nuclear lamin expression in normal testis and testicular germ cell tumours of adolescents and adults." in: The Journal of pathology, Vol. 182, Issue 2, pp. 197-204, 1997 (PubMed).

Pugh, Coates, Lane, Raymond, Quinlan: "Distinct nuclear assembly pathways for lamins A and C lead to their increase during quiescence in Swiss 3T3 cells." in: Journal of cell science, Vol. 110 ( Pt 19), pp. 2483-93, 1997 (PubMed).

Jansen, Machiels, Hopman, Broers, Bot, Arends, Ramaekers, Schouten: "Comparison of A and B-type lamin expression in reactive lymph nodes and nodular sclerosing Hodgkin's disease." in: Histopathology, Vol. 31, Issue 4, pp. 304-12, 1997 (PubMed).

Weaver, Carson, Walker, Chaly, Lach, Raymond, Brown, Sikorska: "Degradation of nuclear matrix and DNA cleavage in apoptotic thymocytes." in: Journal of cell science, Vol. 109 ( Pt 1), pp. 45-56, 1996 (PubMed).

Machiels, Broers, Raymond, de Ley, Kuijpers, Caberg, Ramaekers: "Abnormal A-type lamin organization in a human lung carcinoma cell line." in: European journal of cell biology, Vol. 67, Issue 4, pp. 328-35, 1996 (PubMed).

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