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desert Hedgehog antibody (N-Term)

DHH Reactivity: Mouse WB Host: Rat Monoclonal 6A12 unconjugated
Catalog No. ABIN360880
  • Target See all desert Hedgehog (DHH) Antibodies
    desert Hedgehog (DHH) (Desert Hedgehog (DHH))
    Binding Specificity
    • 7
    • 7
    • 6
    • 6
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 44
    • 14
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Host
    • 45
    • 4
    • 2
    • 2
    Rat
    Clonality
    • 49
    • 4
    Monoclonal
    Conjugate
    • 29
    • 7
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This desert Hedgehog antibody is un-conjugated
    Application
    • 41
    • 23
    • 10
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Specificity
    This antibody detects Mouse DHH N-terminal polypeptide, but not C-terminal one in Western blotting.
    Purification
    Protein A/G affinity chromatography
    Immunogen
    Recombinant dhh N-terminal fragment
    Clone
    6A12
    Isotype
    IgG2
    Top Product
    Discover our top product DHH Primary Antibody
  • Application Notes
    Western Blot: 1/500 - 1/1000.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore with 200 μL sterile PBS and the final concentration is 500 μg/mL.
    Buffer
    0.2 μm filtered PBS solution, None
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    desert Hedgehog (DHH) (Desert Hedgehog (DHH))
    Alternative Name
    Desert Hedgehog / DHH (DHH Products)
    Synonyms
    GDXYM antibody, HHG-3 antibody, SRXY7 antibody, C78960 antibody, Xchh antibody, chh antibody, chh-a antibody, dhh antibody, dhh-1 antibody, dhh-A antibody, hh4 antibody, x-chh antibody, desert hedgehog antibody, desert hedgehog L homeolog antibody, desert hedgehog S homeolog antibody, DHH antibody, dhh antibody, Dhh antibody, dhh.L antibody, dhh.S antibody
    Target Type
    Species
    Background
    Desert Hedgehog (Dhh) belongs to the highly conserved Hedgehog family of proteins which are involved in multiple developmental processes. Hedgehogs are synthesized as 45 kDa precursors that are cleaved autocatalytically. The 19 kDa Nterminal fragment remains membrane associated due to its cholesterol and palmitate modifications. Binding of this fragment to Patched receptors results in the loss of Patched repression of Smoothened signaling. Dhh binds both Patched and Patched 2 as well as Hedgehog interacting protein (Hip). Within the N-terminal peptide, mouse Dhh shares 97 % and 100 % amino acid (aa) sequence identity with human and rat Dhh, respectively. It shares 74 % aa seqeuence identity with mouse Indian (Ihh) and Sonic hedgehog (Shh). Dhh is produced by Sertoli cells and is required for testis development and spermatogenesis. It induces steroidogenic factor 1 which is instrumental in promoting Leydig cell differentiation. It also promotes the deposition of basal lamina surrounding seminiferous tubules. In humans, mutations of Dhh are associated with pure gonadal dysgenesis. Dhh is expressed in the female by ovarian granulosa cells and the corpus luteum. Its upregulation in human ovarian cancer correlates positively with proliferative index and negatively with prognosis. Dhh is also expressed by Schwann cells and is upregulated following nerve injury. It induces the expression of Patched and Hip in nerve fibroblasts and promotes the formation of the connective tissue sheath surrounding peripheral nerves.Synonyms: HHG-3
    Gene ID
    13363
    NCBI Accession
    NP_031883
    UniProt
    Q61488
    Pathways
    Hedgehog Signaling
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